Invitrogen™ HLA-A3 Monoclonal Antibody (GAP.A3), APC, eBioscience™

Catalog No.	Quantity
50-112-3136	100 Tests

Catalog No. 50-112-3136 Supplier Invitrogen™ Supplier No. 17575442

Description

Mouse Monoclonal Antibody

Description: The monoclonal antibody GAP.A3 reacts with the human major histocompatibility complex (MHC) class I molecule HLA-A3, which is a member of the HLA-A family. HLA-A3 exists as two subtypes, HLA-A3.1 and HLA-A3.2, which differ at amino acid positions 152 and 156. MHC class I molecules are expressed on all nucleated cells. These molecules are highly polymorphic, cell surface glycoproteins that non-covalently associate with B2 microglobulin. MHC Class I molecules present intracellular peptides on the cell surface and nonnative peptides are recognized by circulating cytotoxic T lymphocytes. HLA-A3 is expressed by 15-25% of cancer patients and has been demonstrated to bind the tumor-associated antigen human telomerase reverse transcriptase (hTERT). Applications Reported: This GAP.A3 antibody has been reported for use in flow cytometric analysis. Applications Tested: This GAP.A3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

HLA-A3 is a subtype of the HLA class I heavy chain paralogues, existing in two forms: HLA-A3.1 and HLA-A3.2, which differ at amino acid positions 152 and 156. HLA class I molecules are heterodimers consisting of a heavy chain and a light chain (beta-2 microglobulin), with the heavy chain anchored in the membrane. These molecules are highly polymorphic cell surface glycoproteins expressed on nearly all nucleated cells. HLA class I molecules play a central role in the immune system by presenting intracellular peptides derived from the endoplasmic reticulum lumen on the cell surface. Non-native peptides are recognized by circulating cytotoxic T lymphocytes, facilitating immune surveillance. The heavy chain of HLA-A is approximately 45 kDa and its gene contains 8 exons. Exons 2 and 3 encode the alpha1 and alpha2 domains, which bind the peptide, while exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exons 2 and 3 determine the peptide binding specificity of each class I molecule, and typing for these polymorphisms is routinely performed for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described, and HLA and MHC antibodies are crucial in immunopeptidomics, aiding in the identification and characterization of neoantigens through techniques such as high-performance liquid chromatography coupled to tandem mass spectrometry.

Specifications

• Antigen: HLA-A3
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: GAP.A3
• Concentration: 5 μL/Test
• Conjugate: APC
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene: HLA-A
• Gene Accession No.: P04439
• Gene Alias: A29 protein; antigen presenting molecule; Aw-33; Aw-74; HLA A; HLA class I antigen; HLA class I histocompatibility antigen, A alpha chain; HLA class I histocompatibility antigen, A-1 alpha chain; HLA class I histocompatibility antigen, A-30 alpha chain; HLA class I histocompatibility antigen, A-33 alpha chain; HLA class I histocompatibility antigen, A-74 alpha chain; HLA locus; HLAA; HLA-A; HLA-A locus alpha 2 domain; HLA-A null; HLA-A11; HLA-A33; HLA-Aw33.1; HLA-DQB1; HLA-DRB1; Human leukocyte antigen A; leukocyte antigen class I-A; major histocompatibility complex, class I, A; MCH class I antigen; MHC class 1 antigen; MHC class I anti; MHC Class I antigen; MHC class I antigen A*30; MHC class I antigen A*33; MHC class I antigen A*74; MHC class I antigen heavy chain; MHC class I antigen HLA-A heavy chain; MHC class I antigen HLA-A33; MHC class I antigen null protein; MHC class I antigene; MHC class II antigen; MHC classI antigen; truncated MHC class I antigen
• Gene Symbols: HLA-A
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Quantity: 100 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 3105
• Target Species: Human
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2a κ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov