Invitrogen™ HLA-DRB4 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5112826

Catalog No. PIPA5112826

Description

HLA-DRB4 Polyclonal Antibody for Western Blot, IHC (P), ELISA

HLA-DRB4 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB4 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9. HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

Specifications

• Antigen: HLA-DRB4
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: HLA-DRB4
• Gene Alias: DR4; DR-4; DRB1 transplantation antigen; DRB4; HLA class II histocompatibility antigen, DR beta 4 chain; HLA-DR4B; HLA-DRB4; human leucocyte antigen DRB4; leukocyte antigen; major histocompatibility complex, class II, DR beta 4; MHC class II antigen DRB4; MHC class II antigen HLA-DR-beta; MHC class2 antigen; MHC HLA DR-beta chain
• Host Species: Rabbit
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 3126
• Content And Storage: -20°C or -80°C if preferred
• Form: Liquid

• Applications: ELISA, Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.03% ProClin 300; pH 7.4
• Gene Accession No.: P13762
• Gene Symbols: HLA-DRB4
• Immunogen: Recombinant Human HLA class II histocompatibility antigen, DR beta 4 chain protein (30-227AA).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG