Invitrogen™ HLA-E Monoclonal Antibody (MEM-E/02)

Catalog No.	Quantity
01-676-065	100 μg

Catalog No. 01-676-065 Supplier Invitrogen™ Supplier No. MA119309

Description

Mouse Monoclonal Antibody

This antibody reacts with an extracellular epitope on denaturated heavy chain of human HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of all human cell types. Immunohistochemistry (Paraffin): Heat antigen retrieval (sodium citrate), incubation with mAb: 1 hour / RT, detection DAB.

HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

Specifications

• Antigen: HLA-E
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
• Classification: Monoclonal
• Clone: MEM-E/02
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 15mM sodium azide; pH 7.4
• Gene: HLA-E
• Gene Accession No.: P13747
• Gene Alias: A29 protein; antigen presenting molecule; Aw-33; Aw-74; DAMA-277I14.1; DAQB-90C11.16; DKFZp686P19218; EA1.2; EA2.1; FLJ26655; HLA A; HLA class I antigen; HLA class I histocompatibility antigen, A alpha chain; HLA class I histocompatibility antigen, A-1 alpha chain; HLA class I histocompatibility antigen, A-30 alpha chain; HLA class I histocompatibility antigen, A-33 alpha chain; HLA class I histocompatibility antigen, A-74 alpha chain; HLA class I histocompatibility antigen, alpha chain E; HLA class I histocompatibility antigen, E alpha chain; HLA locus; HLA-6.2; HLAA; HLA-A; HLA-A locus alpha 2 domain; HLA-A null; HLA-A11; HLA-A33; HLA-Aw33.1; HLA-DQB1; HLA-DRB1; HLAE; HLA-E; Human leukocyte antigen A; leukocyte antigen class I-A; lymphocyte antigen; major histocompatibility complex, class I, A; major histocompatibility complex, class I, E; MCH class I antigen; MHC; MHC 1; MHC class 1 antigen; MHC class I anti; MHC Class I antigen; MHC class I antigen A*30; MHC class I antigen A*33; MHC class I antigen A*74; MHC class I antigen E; MHC class I antigen heavy chain; MHC class I antigen HLA-A heavy chain; MHC class I antigen HLA-A33; MHC class I antigen null protein; MHC class I antigene; MHC class II antigen; MHC classI antigen; MHC HLA-E alpha-1; MHC HLA-E alpha-2.1; MHC1; QA1; sHLA-E; Soluble HLA class I histocompatibility antigen, alpha chain E; truncated MHC class I antigen
• Gene Symbols: HLA-E
• Host Species: Mouse
• Immunogen: Recombinant human HLA-E denaturated heavy chain.
• Purification Method: Protein A
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 3133
• Target Species: Human, Monkey
• Content And Storage: 4°C, do not freeze
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1