HLA-G Monoclonal Antibody (87G), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-139-20	Invitrogen 14995782	100 μg

Description

Description: The monoclonal antibody 87G recognizes human HLA-G, a member of the Human Leukocyte Antigen family but as part of the nonclassical MHC type involved in inhibiting immune reponses. HLA-G has seven reported isoforms. The antibody 87G recognizes both HLA-G1 and the solube HLA-G5. Expression of HLA-G is found primarily in fetal trophoblast cells as they invade the maternal decidua thereby protecting the fetus from the maternal immune system. Like the highly mitotic trophoblast, abundant HLA-G protein expression has been identified in some tumors, including melanoma, breast carcinoma and renal carcinoma as well as CLL, AML and B-CLL. Some expression has also been found in pancreatic islets, erythroid and endothelial progenitors and the adult thymic medulla.HLA-G+ CD4 or CD8 cells have been identified in normal human peripheral blood and are thought to act as regulatory cells in that they are hypoproliferative with a unique cytokine profile differing from Tregs. The receptors for HLA-G are CD85j/ILT2, CD85d/ILT4, and CD158. Recent studies have shown a role for HLA-G in tolerance and maintece of transplanted organs. Applications Reported: This 87G antibody has been reported for use in flow cytometric analysis, and immunohistology staining of frozen tissue sections. Applications Tested: This 87G antibody has been tested by flow cytometric analysis of normal human peripheral blood. This can be used at less than or equal to 0.5 μg per test.

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

Specifications

• Antigen: HLA-G
• Concentration: 0.5 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: P17693
• Gene Symbols: HLA-G
• Primary or Secondary: Primary
• Content And Storage: 4° C
• Gene: HLA-G

• Clone: 87G
• Applications: Flow Cytometry, Immunohistochemistry (Frozen)
• Conjugate: Unconjugated
• Host Species: Mouse
• Target Species: Human
• Gene ID (Entrez): 3135
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.