Invitrogen™ HLA-G Monoclonal Antibody (MEM-G/1)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA119219

Catalog No. 01-675-983

Description

This antibody reacts with an extracellular epitope of denaturated HLA-G heavy chain. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells. Immunohistochemistry (Paraffin): Incubation: 1 h at RT.

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

Specifications

• Antigen: HLA-G
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 15mM sodium azide; pH 7.4
• Gene Accession No.: P17693
• Gene Symbols: HLA-G
• Immunogen: Recombinant human HLA-G denaturated heavy chain.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1

• Applications: Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
• Clone: MEM-G/1
• Conjugate: Unconjugated
• Gene: HLA-G
• Gene Alias: acrosomal protein SP-10; Acrosomal vesicle protein 1; ACRV1; b2 microglobulin; D11S4365; DADB-15K14.8; HLA class I histocompatibility antigen, alpha chain G; HLA class I molecule; HLA G antigen; HLA-6.0; HLAG; HLA-G; HLA-G histocompatibility antigen, class I, G; LOC768350; major histocompatibility complex, class I, G; MHC class I antigen G; MHC-G; MHCIY; MHC-like class I Y; sHLA-G; Soluble HLA class I histocompatibility antigen, alpha chain G; SP-10; SPACA2; sperm protein 10
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 3135
• Content And Storage: 4°C, do not freeze
• Form: Liquid

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov