Invitrogen™ HLA-G Monoclonal Antibody (MEM-G/9), Biotin

Catalog No.	Quantity
01-676-247	100 μg

Catalog No. 01-676-247 Supplier Invitrogen™ Supplier No. MA119513

Description

Mouse Monoclonal Antibody

This antibody reacts with an extracellular epitope on native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. Reactivity with HLA-G3 was also reported. It will not cross-react with mouse.

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

Specifications

• Antigen: HLA-G
• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation, Immunocytochemistry
• Classification: Monoclonal
• Clone: MEM-G/9
• Concentration: 1 mg/mL
• Conjugate: Biotin
• Formulation: PBS with 15 mM sodium azide; pH 7.4
• Gene: HLA-G
• Gene Accession No.: P17693
• Gene Alias: acrosomal protein SP-10; Acrosomal vesicle protein 1; ACRV1; b2 microglobulin; D11S4365; DADB-15K14.8; HLA class I histocompatibility antigen, alpha chain G; HLA class I molecule; HLA G antigen; HLA-6.0; HLAG; HLA-G; HLA-G histocompatibility antigen, class I, G; LOC768350; major histocompatibility complex, class I, G; MHC class I antigen G; MHC-G; MHCIY; MHC-like class I Y; sHLA-G; Soluble HLA class I histocompatibility antigen, alpha chain G; SP-10; SPACA2; sperm protein 10
• Gene Symbols: HLA-G
• Host Species: Mouse
• Immunogen: Recombinant human HLA-G refolded with beta- 2 Microglobulin and peptide.
• Purification Method: Size-exclusion chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 3135
• Target Species: Human
• Content And Storage: 4°C, do not freeze
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1

Safety and Handling

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