Invitrogen™ HLA-G (soluble) Monoclonal Antibody (5A6G7)

Catalog No.	Quantity
PIMA110357	100 μg

Catalog No. PIMA110357 Supplier Invitrogen™ Supplier No. MA110357

Description

Mouse Monoclonal Antibody

This antibody was generated to a peptide corresponding to C-intron 4-encoded sequence. This antibody does not cross-react with the full-length HLA-G1 isoform and thus allows to distinguish between secreted HLA-G5 and HLA-G6 isoforms from shedded HLA-G1. Purity is >95% by SDS-PAGE. ELISA Positive control: HeLa/HLA-G5 transfectants cell lysate, HeLa/HLA-G5 cell supernatant; negative control: HeLa cell lysate. The antibody 5A6G7 has been tested as the capture antibody in a sandwich ELISA for analysis of soluble HLA-G in combination with antibody W6/32 (Product # MA1-19526). Western blot Positive control: JEG-3 cell lysate, reducing conditions, 12% AA SDS-PAGE. Western Blot: Reducing conditions.

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

Specifications

• Antigen: HLA-G (soluble)
• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: 5A6G7
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 15mM sodium azide; pH 7.4
• Gene: HLA-G
• Gene Accession No.: P17693
• Gene Alias: acrosomal protein SP-10; Acrosomal vesicle protein 1; ACRV1; b2 microglobulin; D11S4365; DADB-15K14.8; HLA class I histocompatibility antigen, alpha chain G; HLA class I molecule; HLA G antigen; HLA-6.0; HLAG; HLA-G; HLA-G histocompatibility antigen, class I, G; LOC768350; major histocompatibility complex, class I, G; MHC class I antigen G; MHC-G; MHCIY; MHC-like class I Y; sHLA-G; Soluble HLA class I histocompatibility antigen, alpha chain G; SP-10; SPACA2; sperm protein 10
• Gene Symbols: HLA-G
• Host Species: Mouse
• Immunogen: C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupled to ovalbumin.
• Purification Method: Protein A
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 3135
• Target Species: Human
• Content And Storage: 4°C, do not freeze
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1