MilliporeSigma™ HMG-CoA Reductase, Mouse, Unlabeled, Clone: IgG-A9,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABS1233

Catalog No. MABS1233

Description

3-hydroxy-3-methylglutaryl-coenzyme A reductase (EC 1.1.1.34; UniProt P00347; also known as HMG-CoA reductase) is encoded by the HMGCR gene (Gene ID 100756363) in Cricetulus griseus (Chinese hamster). HMG-CoA reductase catalyzes the conversion of HMG-CoA to mevalonic acid, the rate-limiting enzyme of the mevalonate-isoprenoid biosynthesis (MIB) pathway responsible for the production of cholesterol and other isoprenoids, as well as farnesyl pyrophosphate (FPP) required for protein prenylation. HMG-CoA reductase is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor as well as oxidized cholesterol species. Competitive inhibitors against HMG-CoA reductase induce LDL receptors expression in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol. HMG-CoA reductase is a multitransmembrane ER protein with an N-terminal sterol-sensing domain (SSD; a.a. 61-218) and a C-terminal catalytic domain (a.a. 450-888) whose activity is inhibited upon SSD binding by cholesterol. Numerous studies have implicated the involvement of HMG-CoA reductase and mevalonate pathway in carcinogenesis.

Specifications

• Antigen: HMG-CoA Reductase
• Classification: Monoclonal
• Concentration: Please refer to lot specific datasheet.
• Formulation: Purified mouse IgG1 in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
• Gene Accession No.: P00347
• Immunogen: Membrane preapration from compactin-adapted CHO cells (Liscum, L., et al. (1983). J. Biol. Chem. 258(13) 8450-8455).
• Quantity: 100 μL
• Research Discipline: Signaling
• Test Specificity: Clone IgG-A9 targets an epitope within HMC-CoA reductase catalytic domain (a.a. 450 887).
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
• Isotype: IgG1 κ

• Applications: Immunoprecipitation, Western Blot
• Clone: IgG-A9
• Conjugate: Unconjugated
• Gene: HMGCR
• Host Species: Mouse
• Purification Method: Protein G Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Hamster
• Form: Purified

For Research Use Only. Not for use in diagnostic procedures.