Invitrogen™ HO-1 Monoclonal Antibody (1F12-A6), APC

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA545152

Catalog No. PIMA545152

Description

1 μg/mL was sufficient for detection of HO-1 in 10 μg of mixed human cell line lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary. Detects 32kDa. Does not cross-react with HO-2.

Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO-1 and a constitutively expressed HO-2. HO-1 is induced by a wide variety of stimuli including conditions of oxidative stress, inflammatory agents, transforming growth factor beta and heat shock. The increase in expression of HO-1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could eventually generate more bilirubin, an anti-oxidant.

Specifications

• Antigen: HO-1
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: 2.48mM MES, 95.64mM phosphate with 0.5M EDTA and no preservative; pH 7.2
• Gene Accession No.: P06762, P09601, P14901, P32394, Q5E9F2
• Gene Symbols: HMOX1
• Immunogen: Human HO-1 synthetic peptide, amino acids 1-30.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Canine, Rabbit, Monkey, Bovine, Hamster, Pig, Guinea Pig
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: 1F12-A6
• Conjugate: APC
• Gene: HMOX1
• Gene Alias: bK286B10; D8Wsu38e; heat shock protein 32; heat shock protein, 32-kD; heme orygenase 1; heme oxygenase; heme oxygenase (decycling) 1; heme oxygenase (decyclizing) 1; heme oxygenase 1; heme oxygenase-1; Hemox; hemoxygenase; Heox; HEOXG; Hmox; Hmox1; HMOX1D; HO; Ho1; HO-1; Hsp32; P32 protein
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 100008919, 100715748, 100756872, 15368, 24451, 3162, 442987, 445512, 513221
• Content And Storage: 4°C
• Form: Liquid