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Invitrogen™ Hoechst 33342 Ready Flow™ Reagent
Description
This dye emits blue fluorescence when bound to double-stranded DNA with an emission maximum at 461 nm.
• Stable at room temperature
• Convenient, ready-to-use format—no need to dilute, weigh, or pipette
• Cell-permeable and effective in fixed and live-cell staining for cell cycle
Hoechst 33342 Ready Flow Reagent is a cell-permeable, blue fluorescent DNA stain for cell cycle analysis. Add 2 drops from the dropper bottle directly into 1 x 106 cells, incubate, and analyze.
Hoechst 33342 reagent binds preferentially to adenine-thymine regions of double-stranded DNA. This dye binds into the minor grooves of DNA and exhibits distinct fluorescence emission spectra that are dependent on dye:base pair ratios. Hoechst 33342 Ready Flow Reagent is excited by UV light and has peak emission at 460 nm.
Specifications
Specifications
| Color | Blue |
| Content And Storage | 2 dropper bottles, store at room temperature |
| Excitation Wavelength Range | 361/497 |
| Dye Type | Cell-Permeant |
| Quantity | 120 assays |
| Product Line | Ready Flow™ |
| Product Type | Nucleic Acid Stain |
| SubCellular Localization | Nucleus |
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