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  5. Novus Biologicals™ HSP27 Antibody Pair [HRP]

Novus Biologicals™ HSP27 Antibody Pair [HRP]
SDP

Catalog No. NB010197 Shop All R&D Systems Products
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NB010197 15 Plates
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Catalog No. NB010197 Supplier Novus Biologicals™ Supplier No. NBP27957915PLATES
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Description

Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human HSP27.

Specifications

Assay Range 9.4 pg/mL
Assay Sensitivity 9.38-600 pg/mL
Assay Sandwich ELISA
Conjugate HRP
Immunoassay Kit Format Sandwich ELISA Kit
Product Type Antibody Pairs
Specificity This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human HSP27.
Target Species Human
For Use With (Application) Sandwich ELISA
Gene Alias 28 kDa heat shock protein, DKFZp586P1322, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, heat shock 27kD protein 1, heat shock 27kDa protein 1, heat shock protein beta-1, HMN2B, HS.76067, Hsp25, HSP27HSP 27, HSP28CMT2F, HspB1, SRP27, Stress-responsive protein 27
Gene ID (Entrez) 3315
Gene Symbol HSPB1
Host Species Mouse/Rabbit
Kit Contents
  • Detection Antibody: 0.5 mg/mL mouse anti-HSP27 monoclonal antibody conjugated to horseradish-peroxidase (HRP).
  • Capture Antibody: 1.0 mg/mL of rabbit anti-HSP27 monoclonal antibody,
  • Standard: Each vial contains 13 ng of recombinant HSP27.
    		•
    Quantity 15 Plates
    Regulatory Status RUO
    Research Discipline Cancer, Cell Biology, Cell Cycle and Replication, Golgi Apparatus Markers, Membrane Trafficking and Chaperones, Neuroscience, Phospho Specific
    Storage Requirements Storage is content dependent.
    Target HSP27
    Technique 1. Capture Antibody: Dilute to a working concentration of 1 μg/mL in CBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 0.6 ng/mL is recommended.
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