Invitrogen™ Human ALDH1A1 ELISA Kit

Description

ELISA

Human ALDH1A1 quantitates human ALDH1A1 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human ALDH1A1.

ALDH1A1 belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme.

Specifications

• Accession Number: acetaldehyde dehydrogenase 1; Ahd2; Ahd-2; alcohol dehydrogenase family 1, subfamily A1; alcohol dehydrogenase family 1, subfamily A2; ALDC; aldehyde dehydrogenase 1 family member A1; aldehyde dehydrogenase 1 family, member A1; aldehyde dehydrogenase 1, liver cytosolic (class 1); aldehyde dehydrogenase 1, soluble; aldehyde dehydrogenase 1, subfamily A1; aldehyde dehydrogenase 1A1; aldehyde dehydrogenase 2; aldehyde dehydrogenase family 1 member A1; aldehyde dehydrogenase family 1, member A1; aldehyde dehydrogenase family 1, subfamily A1; aldehyde dehydrogenase, cytosolic; aldehyde dehydrogenase, liver cytosolic; Aldh; ALDH class 1; Aldh1; ALDH11; ALDH1A1; Aldh1a2; Aldh2; ALDH-E1; ALHDII; E1; epididymis luminal protein 12; epididymis luminal protein 9; epididymis secretory sperm binding protein Li 53 e; HEL12; HEL-9; HEL-S-53 e; MGC2318; PUMB1; RALDH 1; ralDH1; Retinal dehydrogenase 1; retinaldehyde dehydrogenase 1
• Assay Range: 0.4 to 100 ng/mL
• Assay Sensitivity: 0.4 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 216
• Gene Symbol: ALDH1A1
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: ALDH1A1
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov