Abnova™ Human ALK (BAG10812.1, 1058 a.a. - 1620 a.a.) R1275Q mutant Partial Recombinant Protein with GST-tag expressed in Baculovirus infected Sf21 cells

Catalog No.	Quantity
89-933-181	5 μg

Catalog No. 89-933-181 Supplier Abnova™ Supplier No. P5747

Description

Used for Func, SDS-PAGE

The 2;5 chromosomal translocation is frequently associated with anaplastic large cell lymphomas (ALCLs). The translocation creates a fusion gene consisting of the ALK (anaplastic lymphoma kinase) gene and the nucleophosmin (NPM) gene: the 3' half of ALK, derived from chromosome 2, is fused to the 5' portion of NPM from chromosome 5. A recent study shows that the product of the NPM-ALK fusion gene is oncogenic. The deduced amino acid sequences reveal that ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. These sequences are absent in the product of the transforming NPM-ALK gene. ALK shows the greatest sequence similarity to LTK (leukocyte tyrosine kinase). ALK plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. [provided by RefSeq]

Specifications

• Accession Number: BAG10812.1
• For Use With (Application): Functional Study, SDS-PAGE
• Formulation: Liquid
• Gene ID (Entrez): 238
• Molecular Weight (g/mol): 90kDa
• Name: ALK (R1275Q) (Human) Recombinant Protein
• Preparation Method: Baculovirus infected insect cell (Sf21) expression system
• Purification Method: Glutathione sepharose chromatography
• Quality Control Testing: SDS-PAGE Stained with Coomassie Blue
• Quantity: 5 μg
• Storage Requirements: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
• Regulatory Status: RUO
• Gene Alias: CD246/Ki-1/TFG/ALK
• Common Name: ALK
• Gene Symbol: ALK
• Biological Activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
  Substrate : Srctide. ATP: 100 μM.
• Species: Baculovirus
• Recombinant: Recombinant
• Protein Tag: GST
• Expression System: Baculovirus infected insect cell (Sf21) expression system
• Form: Liquid
• Purity or Quality Grade: 66% by SDS-PAGE/CBB staining