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  5. Invitrogen™ Human ALKP ELISA Kit 

Invitrogen™ Human ALKP ELISA Kit

Catalog No. EH19RB
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Description

ELISA

Human ALKP quantitates human ALKP in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human ALKP.

Alkaline phosphatase is a homodimeric protein enzyme (86 kDa), containing two zinc atoms crucial to its catalytic function per monomer, and it is optimally active at alkaline pH environments. The enzyme is found in prokaryotes and eukaryotes, with the same general function but in different structural forms suitable to organism's environment. ALP plays a role in liver metabolism and development within the skeleton. In bacteria, ALP acts as a source of inorganic phosphate. In yeast, ALP can be a useful protein for studying membrane protein sorting.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number Akp2; Akp-2; Alkaline phosphatase 2; alkaline phosphatase 2, liver; alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase precursor (EC 3.1.3.2); alkaline phosphatase tissue non-specific isoform; alkaline phosphatase tissue non-specific isoform/TNS-AP; alkaline phosphatase, biomineralization associated; alkaline phosphatase, liver/bone/kidney; alkaline phosphatase, tissue-nonspecific isozyme; Alpl; APTNAP; AP-TNAP; FLJ40094; FLJ93059; glycerophosphatase; HOPS; liver/bone/kidney isozyme; liver/bone/kidney-type alkaline phosphatase; MGC161443; MGC167935; PHOA; tissue-nonspecific alkaline phosphatase; tissue-nonspecific ALP; Tissue-nonspecific ALP alkaline phosphatase; TNAP; TNSALP; TNS-AP
Assay Range 2 to 500 mIU/mL
Assay Sensitivity 2 mIU/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 249
Gene Symbol ALPL
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target ALPL
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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