Invitrogen™ Human Anti-IFN alpha Antibody ELISA Kit

Description

Includes

Aluminium pouch(es) with a Microwell Plate coated with recombinant human IFN-alpha
HRP-Conjugate human IFN-alpha protein
Human anti-IFN-alpha Standard, 200ng/mL
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Adhesive Films

ELISA

The Human Interferon alpha Antibody (Hu anti-IFNα) ELISA quantitates Hu anti-IFNα in human serum, plasma, urine, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu anti-IFNα. Principle of the method The Human anti-IFNα solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related, species-specific proteins exclusive to vertebrates. The interaction of IFN-alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. In humans, the gene for IFN alpha is located on on chromosome 9. The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities), and single beta, omega, epsilon and kappa isoforms. Homologous molecules are found in many species, including rats and mice (and most mammals), and have been identified in birds, reptiles and fish species. IFN alpha is produced primarily by plasmacytoid dendritic cells, is a potent component of the anti-viral innate immune response and modulates adaptive immunity. Diseases associated with the dysfunction of IFN alpha include Hepatitis C and Hepatitis.

Specifications

• Accession Number: P01562,P01563
• Assay Range: 3.15 to 200 ng/mL
• Assay Sensitivity: 1.4 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant, Urine
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 3439, 3440
• Gene Symbol: IFL,IFN,IFN-ALPHA,IFN-alphaD3,IFNA@,IFNA1,IFN-alphaA,IFNAB,INFA2,IFNA2
• Interassay CV: 4.1%
• Intraassay CV: 3.3
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 20 μL, Serum 20 μL, Supernatant 20 μL, Urine 20 μL
• Storage Requirements: 2°C to 8°C
• Synonym: Anti-alpha interferon, IFN leukocytic, LeIF D
• Target: IFN alpha
• Test Time: 1 hrs 20 mins
• Total Assay Time: 3 hrs 10 mins

Safety and Handling

WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov