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MP Biomedicals™ Human APO A1, Apolipoprotein A-1 ELISA Kit

Catalog No. MP08L100039 Shop All MP Biomedicals, Inc Products
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Description

Quantitative detection of human APOA1/Apolipoprotein A1 in samples of cell culture supernatant, saliva, urine, serum, plasma, cell lysates, tissue homogenates, and other biological fluids.

Human APOA1/Apolipoprotein A1 protein participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase. Defects in APOA1/Apolipoprotein A 1 are a cause of familial hypoalphalipoproteinemia and the low HDL levels as well as amyloidosis van Allen type disease.

  • In this ELISA kit, an APOA1/Apolipoprotein A1 specific antibody has been pre-coated onto 96-well plates and blocked
  • Standards or test samples are added to the wells and subsequently an APOA1/Apolipoprotein A1 specific biotinylated detection antibody is added and followed by washing with wash buffer
  • Afterwards, Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer
  • TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction
  • TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes to yellow after adding an acidic stop solution
  • The density of yellow coloration is directly proportional to the amount of APOA1/Apolipoprotein A1 captured in the plate
  • Sensitivity: 0.188 ng/mL in ELISA assays
TRUSTED_SUSTAINABILITY

Specifications

Assay Sandwich Assay
Conjugate APO A1
Cross Reactivity None
Immunoassay Kit Format 96-well Plate
Label Type Biotin
Product Line MP Biomedicals
Product Type ELISA Kit
Protein Family Apolipoprotein
Sample Type Plasma, Serum, Tissue Homogenates
Specificity No Cross-reactivity to Other Antigens
Substrate TMB
Substrate Properties Chemical Substrate
Substrate Type HRP (Horseradish Peroxidase)
Sufficient For 96-well Plate
Target Species Human
Quantity 96 Preps
Regulatory Status RUO
Storage Requirements 2°C to 8°C
Detection Limit 0.313 ng/mL
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Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

Unless specified otherwise, MP Biomedical™'s products are for research or further manufacturing use only, not for direct human use.

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