Invitrogen™ Human Azurocidin/CAP37/HBP ELISA Kit

Description

ELISA

Human Azurocidin/CAP37/HBP quantitates human Azurocidin/CAP37/HBP in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Azurocidin/CAP37/HBP.

Azurocidin, also called heparin-binding protein (HBP), is an inactive homolog of serine proteinases residing in granulocytes. Initially it was thought to have only activity against gram-negative bacteria. Later it was also found to act against gram-positive bacteria and C. albicans. Azurocidin is considered a family member of polymorphonuclear leukocytes- derived antimicrobial proteins like defensins, LL-37 and lysozyme. Azurocidin has been recognized as a player in the activation and modulation of the immune response and may act to alarm the immune system. The cationic domain at one side of the protein is essential for its antimicrobial activity. It is stored in azurophil granules as well as secretory granules and as a result partly released at early stage of extravasation. Azurocidin is involved in chemotaxis and activation of monocytes, cytokine release and phagocytosis leading to more efficient bacterial clearance. The primary targeted environment of azurocidin are cells in the bloodstream, that is the endothelial lining, and the extravascular surroundings. The interaction of azurocidin with leucocytes is mediated via beta 2-intergrins. High plasma levels of azurocidin might help with the identification of patients which are at risk in developing sepsis.

Specifications

• Accession Number: AZAMP; AZU; AZU1; azurocidin; azurocidin 1; CAP37; cationic antimicrobial protein 37; cationic antimicrobial protein CAP37; HBP; Heparin-binding protein; hHBP; HUMAZUR; NAZC; neutrophil azurocidin
• Assay Range: 0.42 to 100 ng/mL
• Assay Sensitivity: 0.42 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 566
• Gene Symbol: AZU1
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: Azurocidin
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov