Gibco™ Human B7-H2 Fc Recombinant Protein, PeproTech®

Catalog No.	Quantity
310-37-20UG	20 μg
31037100UG	100 μg
310-37-1MG	1 mg
31037250UG	250 μg
31037500UG	500 μg

Catalog No. 310-37-20UG Supplier Gibco™ Supplier No. 3103720UG

Description

Recombinant Protein

PeproTech's CHO cell-derived Recombinant Human B7-H2 Fc is a glycosylated, disulfide-linked homodimer of 942 amino acid residues whose monomer consists of the 238-amino-acid length extracellular portion of B7-H2 fused to the 231-amino-acid length Fc portion of human IgG1 by two glycines. The calculated molecular weight of Recombinant Human B7-H2 Fc dimer is 104.9 kDa; however, due to glycosylation, it migrates at an apparent molecular weight of approximately 90-95 kDa by SDS-PAGE analysis under reducing conditions. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Inducible co-stimulator ligand (ICOSL) is a specific ligand on antigen-presenting cells and cells of the peripheral tissue that binds to the inducible co-stimulator receptor (ICOS). ICOS belongs to the CD28/CD152 receptor family that regulates T-cell activation and function. ICOSL is expressed on monocytes, dendritic cells and B cells and can be induced by inflammatory stimuli in peripheral tissue. Binding to ICOSL delivers a co-stimulatory signal for T cell proliferation and cytokine secretion.

Specifications

• Accession Number: O75144
• For Use With (Application): Functional Assay
• Formulation: protein with no preservative
• Gene ID (Entrez): 23308
• Molecular Weight (g/mol): 104.9 kDa
• Name: Human B7-H2 Fc
• Quantity: 20 μg
• Source: CHO cells
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: AU044799; B7 homolog 2; B7 homologue 2; B7h; B7H2; B7-H2; B7-like protein Gl50; B7-related protein 1; B7RP1; B7RP-1; BG071784; CD275; GI50; GL50; GL50-B; ICOS ligand; ICOSL; ICOS-L; ICOSLG; inducible T-cell costimulator ligand; inducible T-cell co-stimulator ligand; KIAA0653; LICOS; Ly115l; mKIAA0653; RGD1562791; transmembrane protein B7-H2 ICOS ligand
• Common Name: CD275 (B7-H2)
• Gene Symbol: ICOSLG
• Biological Activity: Determined by its ability to induce adhesion in Jurkat cells activated by immobilized anti-CD3. The ED50 for this effect is 0.3-0.6 ug/ml
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: Fc-tag
• Sequence: DTQEKEVRAM VGSDVELSCA CPEGSRFDLN DVYVYWQTSE SKTVVTYHIP QNSSLENVDS RYRNRALMSP AGMLRGDFSL RLFNVTPQDE QKFHCLVLSQ SLGFQEVLSV EVTLHVAANF SVPVVSAPHS PSQDELTFTC TSINGYPRPN VYWINKTDNS LLDQALQNDT VFLNMRGLYD VVSVLRIART PSVNIGCCIE NVLLQQNLTV GSQTGNDIGE RDKITENPVS TGEKNAATGG PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG K
• Content And Storage: -20°C
• Expression System: CHO cells
• Form: Lyophilized
• Purity or Quality Grade: ≥ 95% by SDS-PAGE gel and HPLC analyses.