Invitrogen™ Human B7-H6 His-tag Recombinant Protein

Catalog No.	Quantity
PIRP87955	50 μg
PIRP87956	500 μg

Catalog No. PIRP87955 Supplier Invitrogen™ Supplier No. RP87955

Description

Recombinant Protein

It is recommended that the protein be aliquoted for optimal storage. It is recommended that sterile water (893 μL) be added to the vial to prepare a stock solution of 0.56 mg/mL. Concentration is measured by UV.

B7-H6 (NCR3LG1) is a transmembrane endogenous ligand expressed on the surfaces of tumor cells. B7-H6 binds with NKp30 present on NK cells. Binding of B7-H6 to NKp30 causes the ligation of NKp30 subsequently inducing NK cell activation and target cell cytolysis. B7-H6 is not constitutively expressed on normal tissue. Whether it can be expressed endogenously under certain conditions is under investigation. B7-H6 has been detected on circulating pro-inflammatory CD14(+)CD16(+) monocytes in some patients with sepsis.

Specifications

• Accession Number: Q68D85
• For Use With (Application): Control, ELISA Standard, Binding Assay
• Format: Lyophilized
• Formulation: PBS with 5% mannitol, 5-8% trehalose, 0.01% Tween 80 and no preservative; pH 7.4
• Gene ID (Entrez): 374383
• Molecular Weight (g/mol): 28.1 kDa
• Name: Human B7-H6 His-tag
• pH Range: 7.4
• Purification Method: Purified
• Quantity: 50 μg
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/μg
• Gene Alias: B7 homolog 6; B7H6; B7-H6; DKFZp686I21167; DKFZp686O24166; natural cytotoxicity triggering receptor 3 ligand 1; natural killer cell cytotoxicity receptor 3 ligand 1; NCR3LG1; putative Ig-like domain-containing protein DKFZp686O24166/DKFZp686I21167
• Common Name: B7-H6
• Gene Symbol: NCR3LG1
• Biological Activity: 1.Measured by its binding ability in a functional ELISA. 2.Immobilized human B7-H6 -His (at 10 μg/mL (100 μL/well) can bind human NCR3-Fch, the EC50 of human NCR3-Fch is 6-200 ng/mL)
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: Human B7-H6, amino acids Met1-Ser262 (Accession # NP_001189368.1) with a C-terminal His-tag
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Activity: 1.Measured by its binding ability in a functional ELISA. 2.Immobilized human B7-H6 -His at 10 μg/mL (100 μL/well) can bind human NCR3-Fch, the EC50 of human NCR3-Fch is 6-200 ng/mL.
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: >95% as determined by SDS-PAGE.