Abnova™ Human BRAF (NP_004324.2, 433 a.a. - 726 a.a.) T599_V600insT mutant Partial Recombinant Protein with GST-tag expressed in Baculovirus infected Sf21 cells

Catalog No.	Quantity
89-936-851	5 μg

Catalog No. 89-936-851 Supplier Abnova™ Supplier No. P5756

Description

Used for Func, SDS-PAGE

This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq]

Specifications

• Accession Number: NP_004324.2
• For Use With (Application): Functional Study, SDS-PAGE
• Formulation: Liquid
• Gene ID (Entrez): 673
• Molecular Weight (g/mol): 60kDa
• Name: BRAF (T599_V600insT) (Human) Recombinant Protein
• Preparation Method: Baculovirus infected insect cell (Sf21) expression system
• Purification Method: Glutathione sepharose chromatography
• Quality Control Testing: SDS-PAGE Stained with Coomassie Blue
• Quantity: 5 μg
• Storage Requirements: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
• Regulatory Status: RUO
• Gene Alias: B-RAF1/BRAF1/FLJ95109/MGC126806/MGC138284/RAFB1
• Common Name: BRAF
• Gene Symbol: BRAF
• Biological Activity: The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG (or HRP-labeled anti-mouse IgG).
  Substrate : MAP2K1 [inactive mutant]. ATP: 100 μM.
• Species: Baculovirus
• Recombinant: Recombinant
• Protein Tag: GST
• Expression System: Baculovirus infected insect cell (Sf21) expression system
• Form: Liquid
• Purity or Quality Grade: 55% by SDS-PAGE/CBB staining