Invitrogen™ Human CRP Instant ELISA™ Kit

ELISA

Supplier:  Invitrogen™ BMS288INST

Catalog No. 50-182-38

Description

The Human C-Reactive Protein (Hu CRP) ELISA quantitates Hu CRP in human serum, plasma and cell culture supernatants. The assay will exclusively recognize both natural and recombinant Hu CRP. Principle of the method The Human CRP solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CRP (C-reactive protein) is a major cyclic, pentameric acute phase protein compound consisting of five identical, noncovalently bound, nonglycosylated subunits. Eeach subunit is made up of 206 amino acids, and has a molecular weight of 24 kDa. CRP is produced by the liver, and its plasma levels rise dramatically during inflammatory processes occurring in the body. CRP is an initiator of classical complement cascade, binds to several nuclear components (chromatin, histones, etc.), plays an important role in innate immunity, and mediates activities associated with pre-immune nonspecific host resistance. Specifically, there is a 100-1000 fold increase of CRP in response to injury, infection or inflammation. CRP is opsonic, an initiator of the classical complement cascade, and an activator of monocytes/macrophages. CRP also binds to several nuclear components including chromatin, histones and snRNP, suggesting that it may play a role as a scavenger during cell necrosis. CRP is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host, and initiates pathogen elimination by interacting with humoral and cellular effector systems in the blood. Patients with elevated basal levels of CRP have been shown to be an increased risk for hypertension and cardiovascular disease.

Specifications

• Accession Number: P02741
• Assay Sensitivity: 3.0 pg/mL
• Product Type: ELISA
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: PTX1
• Intraassay CV: 6.9
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: -20°C
• Target: CRP
• Total Assay Time: 2 hrs 10 mins

• Assay Range: 78 to 5,000 pg/mL
• Conjugate: HRP
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 1401
• Interassay CV: 13.1%
• Kit Contents: Pre-coated 96 well Microplate, Detection Antibody, Anti-IgG-HRP, Sample Diluent, lyophilized, Standard (8-well strips x 4), Wash Buffer Concentrate, Substrate Solution, Sample Diluent, Stop Solution, Lysis Buffer 10x, Adhesive Plate Covers
• Quantity: 128 Tests
• Sample Volume: Plasma 10 μL, Serum 10 μL, Supernatant 10 μL
• Synonym: C reactive protein, pentraxin related, PTX1
• Test Time: 15 mins