R&D Systems™ Recombinant Human CCL25/TECK Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR9. The ED50 for this effect is 30-180 ng/mL.

Specifications

• Accession Number: O15444
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Gene ID (Entrez): 6370
• Molecular Weight (g/mol): M.W. (Observed): 14 kDa, reducing conditions; M.W. (theoretical): 14 kDa
• Quantity: 25 μg
• Source: E. coli-derived human CCL25/TECK protein Gln24-Leu150
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: C-C motif chemokine 25, chemokine (C-C motif) ligand 25, Chemokine TECK, Ck beta-15, Ckb15, SCYA25TECKvar, small inducible cytokine subfamily A (Cys-Cys), member 25, Small-inducible cytokine A25, TECK, TECKMGC150327, thymus expressed chemokine, Thymus-expressed chemokine
• Common Name: CCL25/TECK
• Gene Symbol: CCL25
• Biological Activity: Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR9. The ED50 for this effect is typically 30-180ng/mL.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 250 μg/mL in 4 mM HCl.
• Expression System: E. coli-derived Gln24-Leu150
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: CCL25/TECK