Invitrogen™ Human CD19 His-tag Recombinant Protein

Catalog No.	Quantity
PIRP87978	50 μg
PIRP87979	500 μg

Catalog No. PIRP87978 Supplier Invitrogen™ Supplier No. RP87978

Description

Recombinant Protein

It is recommended that the protein be aliquoted for optimal storage. It is recommended that sterile water (200 μL) be added to the vial to prepare a stock solution of 0.25 mg/mL. Concentration is measured by UV.

CD19 is a member of the immunoglobulin superfamily, characterized by two Ig-like domains, and is expressed on B cells throughout all stages of development, excluding terminally differentiated plasma cells. It is also expressed on follicular dendritic cells and has been observed on myeloid leukemia cells, particularly those of monocytic lineage. CD19 is considered the earliest and broadest B cell-restricted antigen, and its expression is found in all B cell precursor leukemias. CD19 forms a multimolecular complex with CD21, CD81, Leu13, MHC class II, and the B cell receptor (BCR), playing a crucial role in B cell signaling. As a signal-amplifying coreceptor for the BCR, CD19 lowers the threshold for antigen receptor-dependent stimulation, allowing B cells to respond specifically and sensitively to various antigens through low-affinity antigen receptors. Signaling through CD19 induces tyrosine phosphorylation, calcium flux, and proliferation of B cells. Beyond its role as a BCR coreceptor, CD19 can also signal independently of BCR co-ligation, serving as a central regulatory component upon which multiple signaling pathways converge. This makes CD19 an important functional regulator of both normal and malignant B cell proliferation. Mutations in the CD19 gene can result in hypogammaglobulinemia, a condition characterized by low levels of immunoglobulins, while CD19 overexpression can lead to B cell hyperactivity. CD19 is expressed on 100% of peripheral B cells, as defined by the expression of kappa or lambda light chains, underscoring its significance in B cell function and immune regulation.

Specifications

• Accession Number: P15391
• Concentration: 0.5 mg/mL
• For Use With (Application): Control, ELISA Standard, Binding Assay
• Format: Lyophilized
• Formulation: PBS with 5% mannitol, 5% trehalose, 0.01% Tween 80 and no preservative; pH 7.4
• Gene ID (Entrez): 930
• Molecular Weight (g/mol): 31.56 kDa
• Name: Human CD19 His-tag
• pH Range: 7.4
• Purification Method: Purified
• Quantity: 50 μg
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/μg
• Gene Alias: AW495831; B4; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Cd19; CD19 antigen; CD19 molecule; CVID3; differentiation antigen CD19; Leu-12; T-cell surface antigen Leu-12
• Common Name: CD19
• Gene Symbol: Cd19
• Biological Activity: Immobilized Human CD19 His (at 2 μg/ml (100 μL/well) can bind Anti-CD19 Antibody, the EC50 of Anti-CD19 Antibody is 8-24 ng/mL)
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: Human CD19, amino acids Met1-Lys291 (Accession # P15391-1) with a C-terminal His-tag
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Activity: Immobilized Human CD19 His at 2 μg/ml (100 μL/well) can bind Anti-CD19 Antibody, the EC50 of Anti-CD19 Antibody is 8-24 ng/mL.
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: ≥90% as determined by SDS-PAGE.