Invitrogen™ Human CD21/CR2/EBV receptor ELISA Kit

Description

ELISA

Human CD21/CR2/EBV receptor quantitates human CD21/CR2/EBV receptor in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human CD21/CR2/EBV receptor.

CD21 (complement receptor 2, CR2, C3D receptor, EBV receptor) binds C3 complement fragments, especially its breakdown fragments, which remain covalently attached to complement activating surfaces or antigen. CD21 has important roles in uptake and retention of immunocomplexes, survival of memory B cells and in development and maintenance of the humoral response to T-dependent antigens. CD21 also serves as a key receptor for Epstein-Barr virus binding and is involved in targeting prions to follicular dendritic cells and expediting neuroinvasion following peripheral exposure to prions. A soluble form of the CD21 (sCD21) is shed from the lymphocyte surface and retains its ability to bind respective ligands. CD21 functions as receptor for C3d, C3dg and iC3b complement components, for EBV and for IFNalpha. CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation. Genetic variations in the CD21 gene are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9). Alternatively, spliced transcript variants encoding different isoforms of CD21 have been found.

Specifications

• Accession Number: C3DR; CD21; CD35; cell surface receptor for C3d; complement C3d receptor; complement C3d receptor 2; complement component (3 b/4 b) receptor 1 (Knops blood group); complement component (3 d/Epstein Barr virus) receptor 2; complement component 3 d receptor 2; complement component receptor 2; complement receptor 1 long isoform; complement receptor 2; complement receptor type 1; Complement receptor type 2; CR; Cr1; Cr-1; Cr2; Cr-2; CVID7; EBV receptor; Epstein-Barr virus receptor; expressed in B lymphocytes; SLEB9
• Assay Range: 17 to 4,000 pg/mL
• Assay Sensitivity: 17 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 1380
• Gene Symbol: CR2
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: CD21
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov