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Human CD25/IL-2R alpha Antibody, R&D Systems™
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Catalog No. MAB623500
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Catalog No. Quantity
MAB623500 500 μg
MAB623SP 25 μg
MAB623100 100 μg
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Description

Mouse Monoclonal Antibody has been used in 3 publications

CD25/IL-2R alpha Monoclonal specifically detects CD25/IL-2R alpha in Human samples. It is validated for Western Blot, Immunohistochemistry, Immunocytochemistry, ELISA Capture (Matched Antibody Pair).

Specifications

Antigen CD25/IL-2R alpha
Applications Western Blot, Immunohistochemistry, Immunocytochemistry, ELISA
Classification Monoclonal
Clone 24204
Conjugate Unconjugated
Dilution Western Blot 1 ug/mL, Immunohistochemistry 8-25 ug/mL, Immunocytochemistry 8-25 ug/mL, ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Gene Accession No. P01589
Gene Alias CD25, CD25 antigen, IDDM10, IL-2 R alpha, IL-2 receptor subunit alpha, IL2R, IL2R alpha, IL-2R subunit alpha, IL-2Ra, IL2RA, IL-2-RA, IL2-RA, interleukin 2 receptor, alpha, interleukin-2 receptor subunit alpha, p55, TAC antigen, TCGFR
Gene Symbols IL2RA
Host Species Mouse
Immunogen S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD25/IL-2 R alpha Glu22-Cys213 Accession # P01589
Purification Method Protein A or G purified from ascites
Quantity 500 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 3559
Test Specificity Detects human CD25/IL-2 R alpha in ELISAs and Western blots. In Western blots, detects recombinant rat CD25/IL-2 R alpha but no cross-reactivity with recombinant mouse CD25/IL-2 R alpha . In sandwich immunoassays, this antibody does not cross-react or interfere with recombinant human (rh) IL-1 RI, rhIL-1 RII, rhIL-2 R beta , rhIL-3 R alpha , rhIL-4 R, rhIL-5 R alpha , rhIL-5 R beta , rhIL-6 R, or rhgp130.
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Target Species Human
Content And Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Form Ascites
Isotype IgG1
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