Invitrogen™ Human CD38 His-tag Recombinant Protein

Description

Recombinant Protein

CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. CD38 functions as a multi-catalytic ectoenzyme serving as ADP-ribosyl cyclase, cyclic ADP-ribose hydrolase and possibly NAD+ glycohydrolase or as a cell surface receptor. Antibodies to CD38 are useful in subtyping of lymphomas and leukemias, detection of plasma cells (i.e. identification of myelomas), and as a marker for activated B and T cells. CD38 participates in cell adhesion, signal transduction and calcium signaling. Further, CD38 is expressed at high levels in the pancreas, liver, kidney, malignant lymphoma and neuroblastoma. Diseases associated with CD38 dysfunction include chronic lymphocytic leukemia and Richter's Syndrome.

Specifications

• Accession Number: P28907
• For Use With (Application): ELISA, Immunohistochemistry, Surface Plasmon Resonance, Western Blot
• Formulation: PBS with 0.01% Tween 80, 5% mannitol, 5% trehalose and no preservative; pH 7.4
• Gene ID (Entrez): 952
• Molecular Weight (g/mol): 31.3 kDa
• Name: Human CD38 His-tag
• Quantity: 50 μg
• Source: HEK293 cells
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/ μg
• Gene Alias: 2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; 2'-phospho-cyclic-ADP-ribose transferase; ADPRC 1; ADPRC1; ADP-ribosyl cyclase 1; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; cADPr hydrolase 1; Cd38; CD38 antigen; CD38 antigen (ADP-ribosyl cyclase / cyclic ADP-ribose hydrolase); CD38 antigen (p45); CD38 antigen p45; CD38 molecule; CD38H; Cd38-rs1; cluster of differentiation 38; cyclic ADP-ribose hydrolase 1; ecto-nicotinamide adenine dinucleotide glycohydrolase; I-19; NAD(+) nucleosidase; NAD+ nucleosidase; NIM-R5 antigen; T10
• Common Name: CD38
• Gene Symbol: CD38
• Biological Activity: 1. Measured by its ability to convert the substrate nicotinamide guanine dinucleotide (NGD+) to cyclic GDPribose.The specific activity is > 2, 500 pmoles/min/μg. 2. Measured by its binding ability in a functional ELISA. Immobilized Human CD38 His-tag Recombinant Protein (at 2 μg/mL (100 μL/well) can bind Mouse Anti-CD38 Antibody, the EC50 of Mouse Anti-CD38 Antibody is 30-120 ng/mL 3.Captured Daratumumab on anti-human IgG Fc via CM5 Chip can bind Human CD38 His-tag Recombinant Protein with an affinity constant of 5.429 nM as determined in a SPR assay (Biacore T200)(Routinely tested).)
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: Human CD38, amino acids Val43-Ile300 (Accession # NP_001766.2) with a C-terminal His-tag
• Content And Storage: -20°Cor to +80°C if preferred
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: >97 % as determined by SDS-PAGE. ≥95 % as determined by SEC-HPLC.