Invitrogen™ Human CD40 Ligand (TNFSF5) Recombinant Protein

Catalog No.	Quantity
PIRP8640	50 μg

Catalog No. PIRP8640 Supplier Invitrogen™ Supplier No. RP8640

Description

Recombinant Protein

CD40L (human) Recombinant Protein, RP-8640, from Invitrogen.

CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2. Recombinant human CD40 Ligand is a non-glycosylated protein, containing 149 amino acids, with a molecular weight of 16.3 kDa. Purity is typically greater than 98% and endotoxin level less than 1 EU/ug. The expected biological activity (ED50) is determined by the dose-dependent production of IL-8 by human PBMCs and is typically 5-10 ng/mL or proliferation of human PBMCs and is typically 1 ug/mL. The specific activity of this protein is 1.3x10^5 units/mg or 1x10^6 units/mg. Reconstitute using sterile water at 0.1mg/mL and centrifuge prior to opening vial. Gently pipet solution down the sides of the vial. DO NOT VORTEX sample. Store reconstituted material at -20°C and add 0.1% BSA for additional stability. Amino acid sequence: MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL.

Specifications

• Accession Number: P29965
• Concentration: 0.1mg/mL
• For Use With (Application): Control
• Formulation: 10mM sodium phosphate with no preservative; pH 7.5
• Gene ID (Entrez): 959
• Molecular Weight (g/mol): 16.3
• Name: Human CD154 (CD40 Ligand)
• pH Range: 7.5
• Quantity: 50 μg
• Source: E. coli
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: H-CD-40-L
• Common Name: Human CD154 (CD40 Ligand)
• Gene Symbol: CD40LG
• Specific Reactivity: Human
• Conjugate: Unconjugated
• Cross Reactivity: Hu
• Species: E. coli
• Recombinant: Recombinant
• Form: Lyophilized
• Protein Subtype: Recombinant
• Purity or Quality Grade: purified

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