Invitrogen™ Human CD96 His-tag Recombinant Protein

Description

Recombinant Protein

It is recommended that the protein be aliquoted for optimal storage. It is recommended that sterile water (400 μL) be added to the vial to prepare a stock solution of 2.5 mg/mL. Concentration is measured by UV.

CD96 is a cell adhesion receptor also known as TACTILE. A member of the Ig superfamily, CD96 is a single pass transmembrane protein consisting of three extracellular Ig-like domains and a proline/serine/threonine rich region. CD96 is expressed on resident monocytes and T cells at all developmental stages (including gamma-delta T cells, thymocytes, and NK cells). In the mouse, this receptor is not expressed on B cells. Unlike in humans, mouse CD96 does not exist as alternative splice variants. Reports indicate that CD96 binds CD155/poliovirus receptor and Nectin-1 to mediate NK cell adhesion to target cells and cytotoxicity.

Specifications

• Accession Number: P40200
• For Use With (Application): ELISA
• Format: Lyophilized
• Formulation: PBS with 0.01% mannitol, 0.01% Tween 80, 5-8% trehalose and no preservative; pH 7.4
• Gene ID (Entrez): 10225
• Molecular Weight (g/mol): 120-130 kDa
• Name: Human CD96 His-tag
• Purification Method: SDS-PAGE
• Quantity: 100 μg
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/μg
• Gene Alias: 1700109I12Rik; Cd96; CD96 antigen; CD96 molecule; cell surface antigen CD96; DKFZp667E2122; sCD96; soluble CD96; T cell activation, increased late expression; t cell-activated increased late expression protein; Tactile; T-cell surface protein tactile
• Common Name: CD96
• Gene Symbol: Cd96
• Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CD155/PVR hFc (at 2 μg/ml (100 μL/well) can bind Human CD96 His, the EC50 of Human CD96 His is 200-800 ng/mL)
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: Human CD96 isoform 2, amino acids Met1-Met503 (Accession # P40200-2) with a C-terminal His-tag
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CD155/PVR hFc (at 2 μg/ml (100 μL/well) can bind Human CD96 His, the EC50 of Human CD96 His is 200-800 ng/mL)
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: >90% as determined by SDS-PAGE.