Abnova™ Human CDC2 (NM_001786, 1 a.a. - 298 a.a.) and CCNE1 (M73812, 1 a.a. - 395 a.a. ) Recombinant Protein with GST-tag expressed in Sf9 cells

Catalog No.	Quantity
89-939-014	100 μg

Catalog No. 89-939-014 Supplier Abnova™ Supplier No. P4655

Description

Used for Func, SDS-PAGE

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available. [provided by RefSeq]

Specifications

• Accession Number: NM_001798 (Gene ID : 1017);M73812 (Gene ID : 898)
• Concentration: 0.243 ug/uL
• For Use With (Application): Functional Study, SDS-PAGE
• Formulation: Liquid
• Gene ID (Entrez): 1017/898
• Molecular Weight (g/mol): CDK2: 60.222kDa, CCNE1
• Name: CDK2/CCNE1 (Human) Recombinant Protein
• Preparation Method: Insect cell (Sf9) expression system
• Purification Method: One-step affinity purification using GSH-agarose
• Quality Control Testing: 2 ug/lane SDS-PAGE Stained with Coomassie Blue
• Quantity: 100 μg
• Storage Requirements: Store at -80°C. Aliquot to avoid repeated freezing and thawing
• Regulatory Status: RUO
• Gene Alias: p33(CDK2)
• Common Name: CDK2
• Gene Symbol: CDK2
• Biological Activity: 241pmol/μg x min
• Species: Baculovirus
• Recombinant: Recombinant
• Protein Tag: GST
• Expression System: Insect cell (Sf9) expression system
• Form: Liquid