Abnova™ Human CDK4 (NM_000075, 4 a.a. - 303 a.a.) and CCND3 (NM_001760, 1 a.a. - 292 a.a.) Recombinant Protein with His tag expressed in Sf9 cells

Catalog No.	Quantity
89-939-033	100 μg

Catalog No. 89-939-033 Supplier Abnova™ Supplier No. P4658

Description

Used for Func, SDS-PAGE

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Specifications

• Accession Number: NM_000075 (Gene ID : 1019);NM_001760 (Gene ID : 896)
• Concentration: 0.337 ug/uL
• For Use With (Application): Functional Study, SDS-PAGE
• Formulation: Liquid
• Gene ID (Entrez): 1019/896
• Molecular Weight (g/mol): CDK4: 59.700kDa, CCND3
• Name: CDK4/CCND3 (Human) Recombinant Protein
• Preparation Method: Insect cell (Sf9) expression system
• Purification Method: One-step affinity purification using GSH-agarose
• Quality Control Testing: 2 ug/lane SDS-PAGE Stained with Coomassie Blue
• Quantity: 100 μg
• Storage Requirements: Store at -80°C. Aliquot to avoid repeated freezing and thawing
• Regulatory Status: RUO
• Gene Alias: CMM3/MGC14458/PSK-J3
• Common Name: CDK4
• Gene Symbol: CDK4
• Biological Activity: 75pmol/μg x min
• Species: Baculovirus
• Recombinant: Recombinant
• Protein Tag: His
• Expression System: Insect cell (Sf9) expression system
• Form: Liquid