Invitrogen™ Human CDK4 Recombinant Protein

Description

Recombinant Protein

This product contains purified recombinant human CDK4 protein with an N-terminal hexahistidine tag. This product may be used as a positive control in many immunological assays.

CDK4/cyclin D3 serine/threonine kinase is dimeric protein complex. CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of cell cycle. CDK4 forms a complex with the D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 can mediate phosphorylation of the C-terminal region of Rb protein leading to an active transcriptional repression of E2F complex. CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivity in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein.

Specifications

• Accession Number: P11802
• Concentration: 0.081 mg/mL
• For Use With (Application): Control, Western Blot
• Formulation: 0.01 M Bis-tris propane with 0.1 M NaCl, 50% glycerol and no preservative; pH 9.0
• Gene ID (Entrez): 1019
• Molecular Weight (g/mol): 38 kDa
• Name: Human CDK4
• pH Range: 9.0
• Quantity: 1 mg
• Source: E. coli
• Immunogen: N/A
• Regulatory Status: RUO
• Gene Alias: Cdk4; Cell division protein kinase 4; CMM3; Crk3; cyclin dependent kinase 4; cyclin-dependent kinase 4; MGC14458; PSK-J3; serine/threonine kinase; Unknown (protein for MGC:133903)
• Common Name: CDK4
• Gene Symbol: CDK4
• Conjugate: Unconjugated
• Species: E. coli
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: Amino acids Met1- Glu303 containing an N-terminal His-tag
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Liquid
• Protein Subtype: Recombinant
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions