Invitrogen™ Human DLL4 Fc Chimera Recombinant Protein

Catalog No.	Quantity
PIRP88043	500 μg
PIRP88042	50 μg

Catalog No. PIRP88043 Supplier Invitrogen™ Supplier No. RP88043

Description

Recombinant Protein

It is recommended that the protein be aliquoted for optimal storage. It is recommended that sterile water (500 μL) be added to the vial to prepare a stock solution of 1 mg/mL. Concentration is measured by UV.

Delta-like ligand 4 (DLL4), also know as Delta-4, is a homologue of the Drosophila delta protein, and functions as a transmembrane bound ligand to the notch receptor Notch1. DLL4 is expressed in the vasculature and plays a critical role in vascular development. It is induced by vascular endothelial growth factor (VEGF), as a negative feedback regulator to regulate angiogenic sprouting and promote the formulation of a differentiated vascular network. DLL4 has been found to be strongly expressed in tumor vessels of clear-cell renal tumors and bladder cancer, and inhibition of DLL4 results in increased vascular proliferation but defective maturation. This in turn leads to a decrease in tumor growth, with no apparent toxicity.

Specifications

• Accession Number: Q9NR61
• Concentration: 0.5 mg/mL
• For Use With (Application): Control, ELISA Standard, Binding Assay
• Format: Lyophilized
• Formulation: PBS with 5% mannitol, 5% trehalose and no preservative; pH 7.4
• Gene ID (Entrez): 54567
• Molecular Weight (g/mol): 81.02 kDa
• Name: Human DLL4 Fc Chimera
• pH Range: 7.4
• Purification Method: Purified
• Quantity: 500 μg
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/μg
• Gene Alias: AOS6; delta 4; delta ligand 4; delta like canonical Notch ligand 4; Delta4; delta-like 4 (Drosophila); delta-like 4 homolog; delta-like 4 protein; delta-like protein 4; DLL4; Drosophila Delta homolog 4; hdelta2; MGC126344; notch ligand delta-2; notch ligand DLL4; UNQ1895/PRO4341
• Common Name: DLL4
• Gene Symbol: Dll4
• Biological Activity: 1. Measured by its binding ability in a functional ELISA. Immobilized Mouse NOTCH1-His (at 2 μg/ml (100 μL/well) can bind human DLL4-hFc, the EC50 of human DLL4-hFc is 15-90 ng/mL 2. Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 1-8 μg/mL in the presence of 500 ng/mL recombinant human BMP2.)
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: Fc-tag
• Sequence: Human DLL4, amino acids Met1-Pro524 (Accession # NP_061947.1) with a C-terminal human IgG1 Fc region
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Activity: 1. Measured by its binding ability in a functional ELISA. Immobilized Mouse NOTCH1-His at 2 μg/ml (100 μL/well) can bind human DLL4-hFc, the EC50 of human DLL4-hFc is 15-90 ng/mL. 2. Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 1-8 μg/mL in the presence of 500 ng/mL recombinant human BMP2.
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: ≥95% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC