Invitrogen™ Human E-selectin (CD62E) Recombinant Protein

Catalog No.	Quantity
PIRP75539	10 μg

Catalog No. PIRP75539 Supplier Invitrogen™ Supplier No. RP75539

Description

Recombinant Protein

RP-75539 contains purified recombinant human e-Selectin protein with an N-terminal hexahistidine tag. RP-75539 may be used as a positive control in many immunological assays.

Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signaling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.

Specifications

• Accession Number: P16581
• Concentration: 0.11 mg/mL
• For Use With (Application): Control, Western Blot
• Formulation: 20 mM tris HCl with 100 mM NaCl, 1 mM EDTA, 50% glycerol and no preservative; pH 8
• Gene ID (Entrez): 6401
• Name: Human E-selectin (CD62E)
• pH Range: 8.0
• Quantity: 10 μg
• Source: E. coli
• Immunogen: N/A
• Regulatory Status: RUO
• Gene Alias: CD62 antigen-like family member E; CD62E; e selectin; Elam; ELAM1; ELAM-1; endothelial adhesion molecule 1; endothelial leukocyte adhesion molecule; endothelial leukocyte adhesion molecule 1; endothelial leukocyte adhesion molecule I; ESEL; E-selectin; LECAM2; leukocyte endothelial cell adhesion molecule 2; leukocyte-endothelial cell adhesion molecule 2; RP1-117P20.2; SELE; selectin E; selectin E (endothelial adhesion molecule 1); selectin, endothelial cell
• Common Name: E-selectin (CD62E)
• Gene Symbol: SELE
• Conjugate: Unconjugated
• Species: E. coli
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: Amino acids Asn179- Leu557 containing an N-terminal His-tag
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Liquid
• Protein Subtype: Recombinant
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions