Invitrogen™ Human EGF Instant ELISA™ Kit

Catalog No.	Quantity
50-246-658	128 Tests

Catalog No. 50-246-658 Supplier Invitrogen™ Supplier No. BMS2070INST

Description

ELISA

The Human Epidermal Growth Factor (Hu EGF) ELISA quantitates Hu EGF in human serum, plasma, cell culture supernatants, or other body fluids. The assay will exclusively recognize both natural and recombinant Hu EGF. Principle of the method The Human EGF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.

Specifications

• Accession Number: P01133
• Assay Range: 3.91 to 250 pg/mL
• Assay Sensitivity: 0.26 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 1950
• Gene Symbol: HOMG4,URG
• Interassay CV: 4.1%
• Intraassay CV: 3.5
• Kit Contents: Pre-coated 96 well Microplate, Biotinylated Detection Antibody, SAV-HRP, Sample Diluent, lyophilized, Standard (8-well strips x 4), Wash Buffer Concentrate, Substrate Solution, Sample Diluent, Stop Solution, Lysis Buffer 10x, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 128 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: -20°C
• Synonym: Epidermal Growth Factor, Urogastrone
• Target: EGF
• Test Time: 15 mins
• Total Assay Time: 3 hrs 30 mins

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov