Invitrogen™ Human EGF Recombinant Protein

Catalog No.	Quantity
PIRP10927	1000 μg

Catalog No. PIRP10927 Supplier Invitrogen™ Supplier No. RP10927

Description

Recombinant Protein

This is a recombinant protein suitable for use as a control in ELISA and Western blot applications. Lyophilized product is stable at room temperature for 3 weeks. Store lyophilized product desiccated at -20°C. Upon reconstitution, store at 4°C for 2-7 days or aliquot and store at -20°C. For long-term storage add either HSA or BSAQ to 0.1%. Avoid repeated freeze-thaw cycles. Reconstitute using distilled water to a concentration not less than 100 μg/mL.

EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.

Specifications

• Accession Number: P01133
• Concentration: 0.1 mg/mL
• For Use With (Application): Control
• Formulation: protein with no preservative
• Gene ID (Entrez): 1950
• Molecular Weight (g/mol): 6.2 kDa
• Name: Human EGF
• Quantity: 1000 μg
• Source: E. coli
• Immunogen: N/A
• Regulatory Status: RUO
• Gene Alias: AI790464; beta-urogastrone; EGF; Epidermal growth factor; epidermal growth factor (beta-urogastrone); epidermal growth factor precursor; H-EGF; HOMG4; Pro-epidermal growth factor; Pro-epidermal growth factor precursor (EGF); sb:eu639; URG; Urogastrone
• Common Name: EGF
• Gene Symbol: EGF
• Biological Activity: 1 x 10^7 Units/mg compared to reference standards
• Conjugate: Unconjugated
• Species: E. coli
• Recombinant: Recombinant
• Sequence: Human EGF recombinant protein contains 53 amino acids
• Content And Storage: -20°C
• Expression System: E. coli
• Form: Lyophilized
• Protein Subtype: Recombinant

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov