Human EGFR Antibody, R&D Systems™

Goat Polyclonal Antibody has been used in 8 publications

Supplier:  R&D Systems AF231

Catalog No. AF231

Description

EGFR Polyclonal specifically detects EGFR in Human samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Immunocytochemistry, CyTOF-ready, ELISA Capture (Matched Antibody Pair).

Specifications

• Antigen: EGFR
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene Accession No.: CAA25240
• Gene Symbols: EGFR
• Immunogen: Mouse myeloma cell line NS0-derived recombinant human EGFR Leu25-Ser645 Accession # CAA25240
• Quantity: 100 μg
• Primary or Secondary: Primary
• Test Specificity: Detects human EGFR in ELISAs and Western blots. In sandwich ELISAs, approximately 3% cross-reactivity with recombinant mouse EGFR is observed and less than 0.1% cross-reactivity with recombinant human (rh) ErbB2 and rhErbB3 is observed.
• Target Species: Human
• Isotype: IgG

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Immunocytochemistry, CyTOF
• Concentration: LYOPH
• Dilution: Western Blot 1 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, Immunohistochemistry 1-15 ug/mL, Immunoprecipitation 1 ug/mL, Immunocytochemistry 1-15 ug/mL, CyTOF-ready, ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
• Gene Alias: avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, EC 2.7.10, EC 2.7.10.1, EGF R, epidermal growth factor receptor, epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog), ErbB, ErbB1, ERBB1PIG61, HER1, HER-1, mENA, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
• Host Species: Goat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Gene ID (Entrez): 1956
• Reconstitution: Reconstitute at 0.2 mg/mL in sterile PBS.
• Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.