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Human EGFR Antibody, R&D Systems™
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Catalog No. AF231
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100 μg
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AF231 100 μg
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Description

Goat Polyclonal Antibody has been used in 8 publications

EGFR Polyclonal specifically detects EGFR in Human samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Immunocytochemistry, CyTOF-ready, ELISA Capture (Matched Antibody Pair).

Specifications

Antigen EGFR
Applications Western Blot, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Immunocytochemistry, CyTOF
Classification Polyclonal
Concentration LYOPH
Conjugate Unconjugated
Dilution Western Blot 1 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, Immunohistochemistry 1-15 ug/mL, Immunoprecipitation 1 ug/mL, Immunocytochemistry 1-15 ug/mL, CyTOF-ready, ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
Gene Accession No. CAA25240
Gene Alias avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, EC 2.7.10, EC 2.7.10.1, EGF R, epidermal growth factor receptor, epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog), ErbB, ErbB1, ERBB1PIG61, HER1, HER-1, mENA, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
Gene Symbols EGFR
Host Species Goat
Immunogen Mouse myeloma cell line NS0-derived recombinant human EGFR Leu25-Ser645 Accession # CAA25240
Purification Method Affinity Purified
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 1956
Test Specificity Detects human EGFR in ELISAs and Western blots. In sandwich ELISAs, approximately 3% cross-reactivity with recombinant mouse EGFR is observed and less than 0.1% cross-reactivity with recombinant human (rh) ErbB2 and rhErbB3 is observed.
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Target Species Human
Content And Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Isotype IgG
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