Gibco™ Human Endostatin Recombinant Protein, PeproTech®

Description

Recombinant Protein

Recombinant Human Endostatin is a 20.2 kDa protein consisting of 184 amino acid residues. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Endostatin is able to avoke non-uniform response for proliferation, cell mount and migration of entothelial cells, which different endostatin binding characteristic, leads to the assumption that endostatin effect is strongly dependent from endothelial cell type. Furthermore endostatin inhibits angiogenesis and tumor growth in vivo by inducing apoptosis in endothelial cells. The local delivery of endostatin seems to specifically affect tumor-associated microvessels by reduction of the vessel density, diameter and functionality. Tumor cell migration and invasion was greatly reduced in the endostatin treated animals. Endostatin is non-toxic and does not induce aquired drug resistance and has therefor become a potent new therapy strategy in solid neoplasias. This therapy appears to have a high potential not only for the treatment of gliomas, the most common brain tumors, but also in other tumors. The ability of endostatin to inhibit neoangiogenesis is mediated, at least in part, by Zn^+2 binding and elastase processing. Widespread endostatin expression was found in elactic fibers in vessel walls and in some other basement membrane zones. Endostatin is released by neurons to accumulate in amyloid plaques in Alzheimer's disease.

Specifications

• Accession Number: P39060
• For Use With (Application): ELISA, Functional Assay, Western Blot
• Formulation: protein with no preservative
• Gene ID (Entrez): 80781
• Molecular Weight (g/mol): 20.2 kDa
• Name: Human Endostatin
• Quantity: 20 μg
• Source: E. coli
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: antiangiogenic agent; Col18a1; Collagen alpha-1(XVIII) chain; collagen type XVIII alpha 1 chain; collagen, type XVIII, alpha 1; endostatin; FLJ27325; FLJ34914; KNO; KNO1; KS; MGC74745; multi-functional protein MFP; NC1; Non-collagenous domain 1; procollagen, type XVIII, alpha 1
• Common Name: Endostatin
• Gene Symbol: COL18A1
• Biological Activity: Measured by its ability to inhibit HUVEC migration.
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: MHSHRDFQPV LHLVALNSPL SGGMRGIRGA DFQCFQQARA VGLAGTFRAF LSSRLQDLYS IVRRADRAAV PIVNLKDELL FPSWEALFSG SEGPLKPGAR IFSFDGKDVL RHPTWPQKSV WHGSDPNGRR LTESYCETWR TEAPSATGQA SSLLGGRLLG QSAASCHHAY IVLCIENSFM TASK
• Content And Storage: -20°C
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.