Gibco™ Human Epiregulin, Animal-Free Recombinant Protein, PeproTech®

Description

Recombinant Protein

Recombinant Human Epiregulin is a 5.6 kDa monomeric protein, containing 50 amino residues, which corresponds to the mature secreted Epiregulin sequence. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Epiregulin is an EGF-related growth factor that binds specifically to EGFR (ErbB1) and ErbB4, but not ErbB2 or ErbB3. It is expressed mainly in the placenta and peripheral blood leukocytes, as well as in certain carcinomas of the bladder, lung, kidney and colon. Epiregulin stimulates the proliferation of keratinocytes, hepatocytes, fibroblasts and vascular smooth muscle cells. It also inhibits the growth of several tumor-derived epithelial cell lines. Human Epiregulin is initially synthesized as a glycosylated 19.0 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce a 6.0 kDa mature secreted sequence.

Specifications

• Accession Number: O14944
• For Use With (Application): Functional Assay
• Formulation: protein with no preservative
• Gene ID (Entrez): 2069
• Molecular Weight (g/mol): 5.6 kDa
• Name: Human Epiregulin, Animal-Free
• Quantity: 500 μg
• Source: E. coli
• Regulatory Status: RUO
• Endotoxin Concentration: <0.1 EU/ μg
• Gene Alias: Ep; Epiregulin; EPR; ER; EREG; Proepiregulin
• Common Name: Epiregulin
• Gene Symbol: Ereg
• Biological Activity: Determined by its ability to stimulate the proliferation of mouse Balb/3T3 cells. The expected ED50 is = 0.2 ng/ml, corresponding to a specific activity of = 5 x 10^6 units/mg
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: MVAQVSITKC SSDMNGYCLH GQCIYLVDMS QNYCRCEVGY TGVRCEHFFL
• Content And Storage: -20°C
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.