Invitrogen™ Human ErbB2 (HER-2) Fc Chimera Recombinant Protein

Description

Recombinant Protein

It is recommended that the protein be aliquoted for optimal storage. It is recommended that sterile water (1695 μL) be added to the vial to prepare a stock solution of 0.83 mg/mL. Concentration is measured by UV.

ErbB2 (HER2) is a receptor tyrosine kinase that is overexpressed in some breast tumors. Herceptin, used in treatment of metastatic Her2-positive cancer, is monoclonal antibody targeting this kinase.

Specifications

• Accession Number: P04626
• Concentration: 0.83 mg/mL
• For Use With (Application): ELISA, Functional Assay
• Format: Lyophilized
• Formulation: PBS with 0.01% Tween 80, 5% mannitol, 5% trehalose and no preservative; pH 7.4
• Gene ID (Entrez): 2064
• Molecular Weight (g/mol): 130-140 kDa
• Name: Human ErbB2 (HER-2) Fc Chimera
• Purification Method: SDS-PAGE
• Quantity: 100 μg
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/ μg
• Gene Alias: avian erythroblastosis oncogene B 2; Avian erythroblastosis viral (v-erb-B2) oncogene homologue 2 (neuro/glioblastoma derived oncogene homolog); CD antigen CD340; CD340; c-erb B2/neu protein; c-erbB2; C-erbB-2; c-neu; epidermal growth factor receptor-related protein; erbb 2; ERBB2; erb-b2; Erbb-2; ErbB2 (pTyr1139); ErbB2 (pY1139); ErbB2 phospho Y1139; erb-b2 receptor tyrosine kinase 2; her 2; HER2; HER-2; HER-2/neu; herstatin; human epidermal growth factor receptor 2; Kiaa3023; metas; Metastatic lymph node gene 19 protein; mKIAA3023; MLN 19; MLN19; NEU; Neu oncogene; NEU proto-oncogene; neuro/glioblastoma derived oncogene homolog; neuroblastoma/glioblastoma derived oncogene homolog; NGL; p185erbB2; p185neu; phospho ErbB2; proto-oncogene c-ErbB-2; Proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene 2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
• Common Name: Her-2 (ErbB2)
• Gene Symbol: ERBB2
• Biological Activity: 1. Immobilized Her2/ERBB2 Protein, Human, Recombinant (ECD, hFc Tag) (at 2 μg/ml (100 μL/well) can bind Anti-Erbb2 Antibody (Trastuzumab), the EC50 is 16-80 ng/mL 2. Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.5-3 μg/mL in the presence of 0.6 μg/mL Anti-ErbB2/Her2 Monoclonal Antibody.)
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: Fc-tag
• Sequence: Human ErbB2, amino acids Met1-Thr652 (Accession # NP_004439.2) with a C-terminal human IgG1 Fc region
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Activity: 1. Immobilized Her2/ERBB2 Protein, Human, Recombinant (ECD, hFc Tag) (at 2 μg/ml (100 μL/well) can bind Anti-Erbb2 Antibody (Trastuzumab), the EC50 is 16-80 ng/mL 2. Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.5-3 μg/mL in the presence of 0.6 μg/mL Anti-ErbB2/Her2 Monoclonal Antibody.)
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: ≥90% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC.