Invitrogen™ Human Fc gamma RIIIA (CD16a) (176 Val) His-tag Recombinant Protein

Catalog No.	Quantity
PIRP87977	1 mg
PIRP87975	100 μg

Catalog No. PIRP87977 Supplier Invitrogen™ Supplier No. RP87977

Description

Recombinant Protein

It is recommended that the protein be aliquoted for optimal storage. It is recommended that sterile water (4000 μL) be added to the vial to prepare a stock solution of 0.25 mg/mL. Concentration is measured by UV.

CD16, known as Fc gamma receptor III, exists in two distinct isoforms: Fc gamma RIIIA and Fc gamma RIIIB. These isoforms are encoded by the genes FCGR3A and FCGR3B, respectively, which are located on chromosome 1 and share a high degree of similarity. Fc gamma RIIIA is expressed as a polypeptide-anchored form on monocytes, macrophages, and lymphocytes such as natural killer (NK) cells, but not on T or B cells. This receptor recognizes the Fc portion of immunoglobulin G (IgG) and is involved in the clearance of immune complexes from circulation. Upon binding IgG, Fc gamma RIIIA activates signal transduction pathways that result in antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, cytokine release, and antigen presentation. Fc gamma RIIIB is expressed on polymorphonuclear neutrophils (PMN) as a glycosylphosphatidylinositol (GPI)-anchored form. It plays a role in similar immune functions, including cellular-mediated cytotoxicity and enhancement of virus infections. Mutations in the FCGR3A gene have been associated with Immunodeficiency 20 and linked to susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. Diseases associated with FCGR3A include Immunodeficiency 20 and Herpes Zoster.

Specifications

• Accession Number: O75015, P08637
• For Use With (Application): Control, ELISA Standard, Binding Assay
• Format: Lyophilized
• Formulation: PBS with 5% mannitol, 5% trehalose, 0.01% Tween 80 and no preservative; pH 7.4
• Gene ID (Entrez): 2214, 2215
• Molecular Weight (g/mol): 44.2 kDa
• Name: Human Fc gamma RIIIA (CD16a) (176 Val) His-tag
• pH Range: 7.4
• Purification Method: Purified
• Quantity: 1 mg
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/μg
• Gene Alias: 4833442P21Rik; CD16; CD16-2; CD16A; CD16a antigen; CD16b; CD32; CD32 receptor 2; CDw32; cytolytic trigger molecule G7; Fc fragment of IgG intermediate affinity IV receptor; Fc fragment of IgG low affinity IIIa receptor; Fc fragment of IgG receptor IIIa; Fc fragment of IgG receptor IIIb; Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor; Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc fragment of IgG, low affinity IIIa, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor for (CD16); Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIb, receptor (CD16b); Fc gamma receptor III; Fc gamma receptor IIIa; Fc gamma receptor III-A; Fc gamma receptor IIIb; Fc gamma RIIIa; Fc gammaRIV; Fc receptor, IgG, low affinity III; Fc receptor, IgG, low affinity IV; Fc receptor-like 3; Fcg receptor III; FCG2; FCG3; Fc-gamma receptor III-2 (CD 16); Fc-gamma receptor IIIb (CD 16); Fc-gamma receptor IIIb (CD16); Fc-gamma RII-b; Fc-gamma RII-c; fc-gamma RIII; Fc-gamma RIIIa; Fc-gamma RIII-alpha; fc-gamma RIIIb; fc-gamma RIII-beta; Fc-gamma-RIIb; Fc-gamma-RIIc; FcgammaRIII; FcgammaRIII a.1; FcgammaRIII a.2; FcgammaRIII a.3; FcgammaRIIIA; FcgammaRIV; FCGR2; FCGR2A; FCGR2B; FCGR2C; FCGR3; Fcgr3a; FCGR3B; Fcgr4; FCGRIII; FcgRIV; FcR-10; FcRII-b; FcRII-c; FcRIII; FCRIIIA; FCRIIIb; Fcrl3; IGFR3; IgG Fc gamma receptor III; igG Fc receptor III; IgG Fc receptor III-1; igG Fc receptor III-2; IMD20; immunoglobulin G Fc receptor III; LOC100911825; Low affinity immunoglobulin gamma Fc region receptor III; low affinity immunoglobulin gamma Fc region receptor III-A; Low affinity immunoglobulin gamma Fc region receptor III-B; low affinity immunoglobulin gamma Fc region receptor III-like; Low affinity immunoglobulin gamma Fc region receptor IV; neutrophil-specific antigen NA; RP11-5K23.1; transmembrane receptor FcgammaRIII-X
• Common Name: CD16
• Gene Symbol: FCGR3A, FCGR3B
• Biological Activity: 1. Using the Octet RED System, the affinity constant (Kd) of CD16a bound to Human IgG1 was 80 nM. 2. Immobilized Human CD16a His (at 2 μg/ml (100 μL/well) can bind Human IgG1, the EC50 of Human IgG1 is 150-450 ng/mL)
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: Human CD16a, amino acids Met1-Gln208 (Accession # P08637-1, with natural variant Phe 176 Val) with a C-terminal His-tag
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Activity: BLI: 1. Loaded Human Fc?RIIIA / CD16a (V176) recombinant protein (Cat. 10389-H08H1) on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 0.83 μM as determined in a BLI assay (Sartorius Octet Red384). 2. Loaded Recombinant Human Fc?RIIIA / CD16a (V176) Protein, His Tag (Cat. No. 10389-H08H1) on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 0.81 μM as determined in BLI assay (Sartorius Octet RED384) (QC tested). SPR: 1. Captured Human Fc?RIIIA / CD16a (V176) recombinant protein (Cat. 10389-H08H1) on AntiHis Chip can bind Bevacizumab (IgG1) with an affinity constant of 0.31 μM as determined in an SPR assay (Biacore T200). 2. Captured Bevacizumab (IgG1) on proA Chip can bind Human Fc?RIIIA / CD16a (V176) recombinant protein (Cat. 10389- H08H1) with an affinity constant of 0.78 μM as determined in an SPR assay (Biacore T200).
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: >95% as determined by SDS-PAGE.