Invitrogen™ Human Ficolin-3 ELISA Kit

Description

ELISA

Human Ficolin-3 quantitates human Ficolin-3 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Ficolin-3.

H-Ficolin (Hakata antigen, Ficolin-3) is an innate immunity pattern recognition molecule found in human serum. H-Ficolin binds to distinct pathogen-associated molecular patterns (PAMP) formed by carbohydrates (GlcNAc, GalNAc and fucose) activating complement through the lectin pathway by means of the associated MASPs ((MBL)-associated serine proteases). H-Ficolin consists of 35 kDa protein chains associated in triplet subunits which are again associated in high molecular weight oligomers by means of disulfide bonds at the N-terminal region.

Specifications

• Accession Number: Collagen/fibrinogen domain-containing lectin 3 p35; Collagen/fibrinogen domain-containing protein 3; FCN3; FCNH; ficolin 3; Ficolin-3; HAKA1; Hakata antigen; MGC22543
• Assay Range: 0.25 to 50 ng/mL
• Assay Sensitivity: 0.25 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 8547
• Gene Symbol: FCN3
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: Ficolin 3
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins