Gibco™ Human FRP-5 (soluble) Recombinant Protein, PeproTech®

Description

Recombinant Protein

PeproTech's CHO cell-derived Recombinant Human sFRP5 is a 288-amino-acid length glycoprotein with a calculated molecular weight of 32.7 kDa; however, due to glycosylation, protein migration occurs at an apparent molecular weight of approximately 33-35 kDa by SDS-PAGE analysis under both reducing and non-reducing conditions. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Secreted frizzled-related protein 5 (SFRP5) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas.

Specifications

• Accession Number: Q5T4F7
• For Use With (Application): Functional Assay
• Formulation: protein with no preservative
• Gene ID (Entrez): 6425
• Molecular Weight (g/mol): 32.7 kDa
• Name: Human FRP-5 (soluble)
• Quantity: 1 mg
• Source: CHO cells
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: AI605071; Frizzled-related protein 1 b; FRP1B; FRP-1 b; SARP3; SARP-3; secreted apoptosis related protein 3; secreted apoptosis-related protein 3; secreted frizzled related protein 5; secreted frizzled-related protein 5; secreted frizzled-related sequence protein 5; SFRP5; sFRP-5
• Common Name: SFRP5
• Gene Symbol: SFRP5
• Biological Activity: Determined by its ability to decrease alkaline phosphatase activity in CCL-226 cells when treated with 25 ng/ml of Murine Wnt-3 a.
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: EEYDYYGWQA EPLHGRSYSK PPQCLDIPAD LPLCHTVGYK RMRLPNLLEH ESLAEVKQQA SSWLPLLAKR CHSDTQVFLC SLFAPVCLDR PIYPCRSLCE AVRAGCAPLM EAYGFPWPEM LHCHKFPLDN DLCIAVQFGH LPATAPPVTK ICAQCEMEHS ADGLMEQMCS SDFVVKMRIK EIKIENGDRK LIGAQKKKKL LKPGPLKRKD TKRLVLHMKN GAGCPCPQLD SLAGSFLVMG RKVDGQLLLM AVYRWDKKNK EMKFAVKFMF SYPCSLYYPF FYGAAEPH
• Content And Storage: -20°C
• Expression System: CHO cells
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.