Applied Biosystems™ Human GAPD (GAPDH) Endogenous Control (VIC™/TAMRA™ probe, primer limited)

Catalog No.	Quantity
43-108-84E	2500 reactions

Catalog No. 43-108-84E Supplier Applied Biosystems™ Supplier No. 4310884E

Description

Human GAPD (GAPDH) Endogenous Control (VIC™/TAMRA™ probe, primer limited)

The Applied Biosystems™ Human GAPD (GAPDH) Endogenous Control (VIC™ ⁄ TAMRA Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other TaqMan™ gene expression assays. Probe is labeled with VIC™ dye - TAMRA™ dye and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Made to Order TaqMan™ Gene Expression Assays, Custom TaqMan™ Gene Expression Assays, and⁄or Custom TaqMan™ Primers and Probes.

Assay Details:
Gene Symbol: GAPDH
RefSeq: NM_002046.3
Probe Exon Location: 3
Amplicon Size: 118

TaqMan™ Endogenous Controls
Eliminate months of assay design, formulation, and testing by using TaqMan™ Endogenous Controls as your controls to quantify gene expression. This convenient collection of pre-designed probe and primer sets enables you to normalize the amount of sample RNA or DNA in a reaction.

Complete Solution for Quantitative Gene Expression
Having a hard time deciding what controls to use to quantify gene expression — even with detailed information on biological systems? Now, with TaqMan™ Endogenous Controls, you can avoid all the trial-and-error of selecting controls for most common human, mouse, rat, and eukaryotic genes.

Simple to Use
All components of the TaqMan™ Endogenous Controls are QC tested, formulated into a single 20X mix, and functionally tested. The controls are not only simple to use, but they are also fully compatible with universal conditions for two-step RT-PCR. Just add TaqMan™ Universal PCR Master Mix (with or without AmpErase™ UNG) and your cDNA sample to generate sensitive, reproducible, and truly quantitative gene expression data on Applied Biosystems instruments including the Applied Biosystems 7900HT, 7300, 7500 Real-Time PCR Systems, and the 7000 and 7700 Sequence Detection Systems.

Flexible Offering
We build each endogenous control using our proven 5' nuclease chemistry. For maximum flexibility, you can choose between two different reporter dyes and two quenchers:

A FAM™ dye-labeled TaqMan™ MGB probe (250nM, final concentration) and two unlabeled PCR primers (900nM each)

A VIC™ dye-labeled TaqMan™ MGB probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)

A VIC™ dye-labeled TAMRA™ probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)

Choosing the Right Endogenous Control
Endogenous controls can normalize the expression levels of target genes by correcting differences in the amount of cDNA that is loaded into PCR reaction wells. For best results, verify that the endogenous control is consistently expressed in the sample set to be tested. Endogenous control expression must be uniform across all samples in the study. For multiplexing, ensure that the gene expression level of the endogenous control is greater than that of the target.

Multiplex vs. Singleplex PCR
All TaqMan™ Endogenous Controls that contain probes labeled with the VIC™ reporter dye are primer limited. This allows multiplexing of TaqMan™ Endogenous Controls with target gene expression assays, provided that the control gene is more abundantly expressed than the target gene. All TaqMan™ Endogenous Controls that contain probes labeled with the FAM™ reporter dye are not primer limited and are not intended for multiplexing .

For Research Use Only. Not for use in diagnostic procedures.

Specifications

• Concentration: 20X
• Accession Number: NM_002046.3
• Amplicon Size: 118
• Content And Storage: Control Primer-Probe Set(s)
• Detection Method: Primer-probe
• Form: Liquid
• Format: Tube
• PCR Method: 2-step RT-qPCR
• Gene Symbol: GAPDH
• Label or Dye: VIC
• Product Line: TaqMan, VIC
• Quantity: 2500 reactions
• Shipping Condition: Dry Ice
• Species: Human
• Target: GAPDH
• For Use With (Application): Gene Expression
• No. of Reactions: 2500 Reactions

Frequently Asked Questions (FAQs)

Can TaqMan Gene Expression Assays be used in a multiplex reaction?

Yes. For duplexing, we have VIC-labeled probes with primer limited concentrations (VIC PL) available from our pre-designed assay selection. The primer limited assay is used to detect the most abundant gene. For guidance on multiplexing, see the TaqMan Multiplex PCR Optimization User Guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/taqman_optimization_man.pdf).

How can I calculate the concentration of a TaqMan probe?

The initial concentration or total yield of your synthesized probe is listed on the data sheet supplied with the probe. TaqMan probes are sometimes shipped in the lyophilized state, but more often are shipped in solution (1X TE). To create a working stock solution of your probe or primers, you should reconstitute or dilute the probe in the appropriate volume of sterile 1X TE (1 mM Tris, 0.1 mM EDTA, pH 8.0) or sterile, nuclease-free H2O. Detailed instructions on how to calculate the proper volume of solution to add to your probe are provided in our online Tutorial document "Reconstituting and Diluting Primers and TaqMan Probes". You can find a copy on our website by entering this title in the main Search field.

Why does a TaqMan TAMRA probe need to have more C's (Cytosines) than G's (Guanidines) in the sequence?

Empirical data has shown that TaqMan TAMRA probes which contain more Gs than Cs will generally have lower ΔRn values and overall poor performance compared to those that do not. For more information on designing TaqMan TAMRA probes, please consult tutorials related to the Primer Express software.

For Research Use Only. Not for use in diagnostic procedures.