Invitrogen™ Human Granzyme B ELISA Kit

Description

ELISA

Granzyme B is a member of the granzyme serine protease family, and is found in the granules of cytotoxic T cells and NK cells. Granzyme B has been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation, and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. Granzyme B is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved in this process has been identified as mannose 6-phosphate receptor which functions as a death receptor for Granzyme B during cytotoxic T cell-induced apoptosis. Granzyme B enters target cells to cleave caspase-3 and initiate the caspase cascade leading to DNA fragmentation and apoptosis. Granzyme B can also act through a mitochondrial apoptosis pathway by cleaving the Bid protein. Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes (A to G) have been isolated and cloned from mouse CTLs and NK cells, however in man, fewer have been cloned and identified.

Specifications

• Accession Number: P10144
• Assay Range: 0.7 to 480 pg/mL
• Assay Sensitivity: <0.43 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 3002
• Gene Symbol: GZMB
• Interassay CV: 6.8%
• Intraassay CV: 6.2
• Kit Contents: Pre-coated 96 well plate, Standard, Detection antibody (monoclonal antibody to human Granzyme B), Biotin-Conjugate anti-human Granzyme B monoclonal antibody, Streptavidin-HRP, human Granzyme B Standard lyophilized, Wash Buffer Concentrate 20x, Dilution Buffer Concentrate 5x, Substrate Solution, Stop Solution, Adhesive Films
• Label or Dye: HRP
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: Granzyme B
• Test Time: 1 hrs
• Total Assay Time: 2 hrs 30 mins