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Human HB-EGF Antibody, R&D Systems™
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Catalog No. AF259SP
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25 μg
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AF259SP 25 μg
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Description

Goat Polyclonal Antibody

HB-EGF Polyclonal specifically detects HB-EGF in Human samples. It is validated for Western Blot, Immunohistochemistry, Neutralization, ELISA Capture (Matched Antibody Pair).

Specifications

Antigen HB-EGF
Applications Western Blot, Immunohistochemistry, Neutralization, ELISA
Classification Polyclonal
Concentration LYOPH
Conjugate Unconjugated
Dilution Western Blot 0.1 ug/mL, Immunohistochemistry 5-15 ug/mL, Neutralization 0.1-0.6 ug/mL, ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Gene Accession No. Q99075
Gene Alias diphtheria toxin receptor (heparin-binding epidermal growth factor-like growthfactor), Dtr, DTRHEGFLdiphtheria toxin receptor (heparin-binding EGF-like growth factor), Dts, DTSF, HBEGF, Hegfl, heparin-binding EGF-like growth factor, heparin-binding epidermal growth factor, proheparin-binding EGF-like growth factor
Gene Symbols HBEGF
Host Species Goat
Immunogen S. frugiperda insect ovarian cell line Sf 21-derived recombinant human HB-EGF (R&D Systems, Catalog # 259-HE) Asp63-Leu148 Accession # Q99075
Purification Method Affinity Purified
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 1839
Test Specificity Detects human HB-EGF in ELISAs and Western blots. In direct ELISAs, less than 1% cross reactivity with recombinant mouse HB-EGF is observed. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) Amphiregulin, rhBetacellulin, rhEpiregulin, and recombinant mouse Epigen is observed.
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Target Species Human
Content And Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Isotype IgG
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