Gibco™ Human HGF Recombinant Protein, PeproTech®

Description

Recombinant Protein

100-39-1MG will be provided as 4 x 250 μg (100-39-250UG). 100-39-500UG will be provided as 2 x 250 μg (100-39-250UG). Recombinant Human HGF is an 80.0 kDa polypeptide consisting of 697 amino acid residues. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms.

Specifications

• Accession Number: P14210
• For Use With (Application): Functional Assay
• Formulation: protein with no preservative
• Gene ID (Entrez): 3082
• Molecular Weight (g/mol): 80 kDa
• Name: Human HGF
• Quantity: 100 μg
• Source: Insect cells
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/μg
• Gene Alias: C230052L06Rik; DFNB39; Fibroblast derived tumor cytotoxic factor; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor; Hepatocyte growth factor (hepapoietin A; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatocyte growth factor alpha chain; Hepatocyte growth factor beta chain; Hepatopoeitin A; hepatopoeitin-A; HepatopoietinA; hepatopoietin-A; Hgf; HGF/SF; HGFB; HPTA; Lung fibroblast derived mitogen; lung fibroblast-derived mitogen; NK1; NK2; Scatter factor; scatter factor); SF; SF/HGF
• Common Name: HGF
• Gene Symbol: Hgf
• Biological Activity: Determined by the dose-dependent stimulation of the proliferation of monkey 4 MBr-5 cells. The expected ED50 for this effect is 1.0-20.0 ng/ml
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: Alpha chain: QRKRRNTIHE FKKSAKTTLI KIDPALKIKT KKVNTADQCA NRCTRNKGLP FTCKAFVFDK ARKQCLWFPF NSMSSGVKKE FGHEFDLYEN KDYIRNCIIG KGRSYKGTVS ITKSGIKCQP WSSMIPHEHS FLPSSYRGKD LQENYCRNPR GEEGGPWCFT SNPEVRYEVC DIPQCSEVEC MTCNGESYRG LMDHTESGKI CQRWDHQTPH RHKFLPERYP DKGFDDNYCR NPDGQPRPWC YTLDPHTRWE YCAIKTCADN TMNDTDVPLE TTECIQGQGE GYRGTVNTIW NGIPCQRWDS QYPHEHDMTP ENFKCKDLRE NYCRNPDGSE SPWCFTTDPN IRVGYCSQIP NCDMSHGQDC YRGNGKNYMG NLSQTRSGLT CSMWDKNMED LHRHIFWEPD ASKLNENYCR NPDDDAHGPW CYTGNPLIPW DYCPISRCEG DTTPTIVNLD HPVISCAKTK QLR. Beta chain: VVNGIPTRTN IGWMVSLRYR NKHICGGSLI KESWVLTARQ CFPSRDLKDY EAWLGIHDVH GRGDEKCKQV LNVSQLVYGP EGSDLVLMKL ARPAVLDDFV STIDLPNYGC TIPEKTSCSV YGWGYTGLIN YDGLLRVAHL YIMGNEKCSQ HHRGKVTLNE SEICAGAEKI GSGPCEGDYG GPLVCEQHKM RMVLGVIVPG RGCAIPNRPG IFVRVAYYAK WIHKIILTYK VPQS.
• Content And Storage: -20°C
• Expression System: Insect cells
• Form: Lyophilized
• Purity or Quality Grade: ≥98% by SDS-PAGE gel and HPLC analyses.