R&D Systems™ Recombinant Human His6-USP11 Protein, CF

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Reaction conditions will need to be optimized for each specific application. We recommend an initial recombinant human USP11 concentration of 10-50 nM when using Ubiquitin-AMC or Ubiquitin-Rhodamine substrates (U-550, U-555).

Specifications

• Accession Number: P51784
• For Use With (Application): Bioactivity
• Formulation: X mg/mL (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
• Gene ID (Entrez): 8237
• Molecular Weight (g/mol): M.W. (theoretical): 111 kDa
• Name: USP47
• Quantity: 50 μg
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP11 protein Contains an C-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Gene Alias: Deubiquitinating enzyme 11, EC 3.1.2.15, EC 3.4.19.12, ubiquitin carboxyl-terminal hydrolase 11, ubiquitin specific peptidase 11, ubiquitin specific protease 11, ubiquitin thioesterase 11, Ubiquitin thiolesterase 11, ubiquitin-specific processing protease 11, Ubiquitin-specific-processing protease 11, UHX1, UHX1ubiquitin carboxyl-terminal hydrolase, X-linked
• Common Name: USP11
• Gene Symbol: USP11
• Biological Activity: Reaction conditions will need to be optimized for each specific application. We recommend an initial recombinant human USP11 concentration of 10-50 nM when using Ubiquitin-AMC or Ubiquitin-Rhodamine substrates (U-550, U-555).
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Expression System: Spodoptera frugiperda, Sf 21 (baculovirus)-derived Contains an C-terminal 6-His tag
• Purity or Quality Grade: >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain.
• Protein: USP11