R&D Systems™ Recombinant Human His6-USP12 Protein, CF

Catalog No.	Quantity
E578050	50 μg

Catalog No. E578050 Supplier R&D Systems™ Supplier No. E578050

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Recombinant Human USP12 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial USP12 concentration of 50-500 nM. Requires a 1:1 stoichiometric amount of UAF1 (E-566) for activity when using Ubiquitin-AMC or Ubiquitin-Rhodamine (U-550, U-555) as a substrate. In vitro, the USP12/UAF1 complex will not efficiently hydrolyze poly-Ubiquitin chains in the absence of WDR20.

Specifications

• Accession Number: O75317
• For Use With (Application): Bioactivity
• Formulation: X mg/mL (X μM) in 50 mM HEPES pH 8.2, 200 mM NaCl, 10% (v/v) Glycerol, 2 mM TCEP
• Gene ID (Entrez): 219333
• Molecular Weight (g/mol): M.W. (theoretical): 44 kDa
• Quantity: 50 μg
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP12 protein Contains a C-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Gene Alias: Deubiquitinating enzyme 12, EC 3.1.2.15, EC 3.4.19.12, UBH1, UBH1ubiquitin specific protease 12, ubiquitin carboxyl-terminal hydrolase 12, ubiquitin specific peptidase 12, ubiquitin specific protease 12 like 1, ubiquitin thioesterase 12, Ubiquitin thiolesterase 12, Ubiquitin-hydrolyzing enzyme 1, Ubiquitin-specific-processing protease 12, USP12L1
• Common Name: USP12
• Gene Symbol: USP12
• Biological Activity: Recombinant Human USP12 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial USP12 concentration of 50-500 nM. Requires a 1:1 stoichiometric amount of UAF1 (E-566) for activity when using Ubiquitin-AMC or Ubiquitin-Rhodamine (U-550, U-555) as a substrate. In vitro, the USP12/UAF1 complex will not efficiently hydrolyze poly-Ubiquitin chains in the absence of WDR20.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Expression System: Spodoptera frugiperda, Sf 21 (baculovirus)-derived Contains a C-terminal 6-His tag
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain.
• Protein: USP12