Invitrogen™ Human ICAM-1 (CD54) His-tag Recombinant Protein

Catalog No.	Quantity
PIRP88058	50 μg
PIRP88060	1 mg

Catalog No. PIRP88058 Supplier Invitrogen™ Supplier No. RP88058

Description

Recombinant Protein

It is recommended that the protein be aliquoted for optimal storage. It is recommended that sterile water (700 μL) be added to the vial to prepare a stock solution of 1.43 mg/mL. Concentration is measured by UV.

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.

Specifications

• Accession Number: P05362
• For Use With (Application): Control, Bioactivity
• Format: Lyophilized
• Formulation: PBS with 5% mannitol, 5% trehalose, 0.01% Tween 80 and no preservative; pH 7.4
• Gene ID (Entrez): 3383
• Molecular Weight (g/mol): 50.96 kDa
• Name: Human ICAM-1 (CD54) His-tag
• pH Range: 7.4
• Purification Method: Purified
• Quantity: 50 μg
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: BB2; CD54; cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM; Icam1; ICAM-1; ICAM-1; CD54 homolog; Intercellular adhesion molecule 1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule-1; intercellular adhesion molecule-1 precursor; Ly 47; Ly-47; major group rhinovirus receptor; MALA2; MALA-2; MyD10; P3.58; sCD54; soluble CD54; Surface antigen of activated B cells
• Common Name: ICAM-1 (CD54)
• Gene Symbol: ICAM1
• Biological Activity: Measured by the ability of the immobilized protein to support the adhesion of PMA-stim μLated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to ICAM1-coated plates (12.5 μg/ml, 100 μL/well), approximately 30%-60% will adhere specifically.
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: Met1-Glu480
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Activity: Measured by the ability of the immobilized protein to support the adhesion of PMA-stim μLated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to ICAM1-coated plates (12.5 μg/ml, 100 μL/well), approximately 30%-60% will adhere specifically.
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: >98% as determined by SDS-PAGE.