Invitrogen™ IFN alpha Human Instant ELISA™ Kit

Instant ELISA Kit

Supplier:  Invitrogen™ BMS216INST

Catalog No. 50-180-29

Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human IFN-alpha, HRP-Conjugate (human IFN-alpha monoclonal antibody) and Assay Buffer, lyophilized
Aluminum pouch(es) with a human IFN-alpha Standard curve (coloured)
Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
Assay Buffer Concentrate 20x (Use when an external predilution of the samples is needed)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films

Description

The Human Interferon alpha(Hu IFNα) ELISA quantitates Hu IFNα in human serum, plasma, cell culture supernatants or other body fluids. The assay will exclusively recognize both natural and recombinant Hu IFNα. Principle of the method The Human IFNα solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related, species-specific proteins exclusive to vertebrates. The interaction of IFN-alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. In humans, the gene for IFN alpha is located on on chromosome 9. The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities), and single beta, omega, epsilon and kappa isoforms. Homologous molecules are found in many species, including rats and mice (and most mammals), and have been identified in birds, reptiles and fish species. IFN alpha is produced primarily by plasmacytoid dendritic cells, is a potent component of the anti-viral innate immune response and modulates adaptive immunity. Diseases associated with the dysfunction of IFN alpha include Hepatitis C and Hepatitis.

Specifications

• Accession Number: P01562,P01563
• Assay Sensitivity: 3.3 pg/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: IFL,IFN,IFN-ALPHA,IFN-alphaD3,IFNA@,IFNA1,IFN-alphaA,IFNAB,INFA2,IFNA2
• Intraassay CV: 6%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: -20°C
• Target: Human
• Total Assay Time: 2 hr. 10 min.

• Assay Range: 39-2,500 pg/mL
• Conjugate: HRP
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 3439, 3440
• Interassay CV: 7.4%
• Kit Contents: Pre-coated 96 well Microplate, Detection Antibody, Anti-IgG-HRP, Sample Diluent, lyophilized, Standard curve strips, Wash Buffer Concentrate, Substrate Solution, Stop Solution, Lysis Buffer 10x, Adhesive Plate Covers
• Quantity: 128 Tests
• Sample Volume: Plasma, 20 μL; Serum, 20 μL; Supernatant, 20 μL
• Synonym: Alpha interferon, IFN leukocytic, LeIF D
• Test Time: 20 min.

For Research Use Only